Results 51 to 60 of about 210,987 (303)

Analysis of the proteome and the secretome of animal trypanosomes : a standardized analytical method to look for new molecular targets [PDF]

, 2007
The causative agents of animal trypanosomosis are various species of protozoan parasites belonging to the genus Trypanosoma, among which T. congolense and T. evansi are the major pathogenic species. The extra cellular position of the trypanosomes implies
Bengaly, Zakaria, Boulangé, Alain, Brizard, Jean-Paul, Courtois, Pierrette, Cuny, Gérard, Daulouède, Sylvie, Frutos, Roger, Grébaut, Pascal, Holzmuller, Philippe, Peltier, Jean-Benoît, Vincendeau, Philippe   +10 more
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Variation of proteomic profile during lactation in Girgentana goat milk: a preliminary study

Italian Journal of Animal Science, 2019
The knowledge of milk proteome has been greatly enhanced by technological advances in the proteomics field as the use of the two-dimensional differential in-gel electrophoresis, a gel-based approach which allowed the analysis of proteins from complex ...
Rosalia Di Gerlando, Marco Tolone, Anna Maria Sutera, Giuseppina Monteleone, Baldassare Portolano, Maria Teresa Sardina, Salvatore Mastrangelo   +6 more
doaj   +1 more source

Preparative free-solution isotachophoresis for separation of human plasma lipoproteins: apolipoprotein and lipid composition of HDL subfractions

Journal of Lipid Research, 2000
We have previously shown that plasma lipoproteins can be separated by analytical capillary isotachophoresis (ITP) according to their electrophoretic mobility in a defined buffer system.
Alfred Böttcher, Judith Schlosser, Florian Kronenberg, Hans Dieplinger, Gabriele Knipping, Karl J. Lackner, Gerd Schmitz   +6 more
doaj   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Influence of cooking process on protein fractions in cooked ham and mortadella

Italian Journal of Animal Science, 2011
The mortadella is a pork meat sausage (in natural or artificial bowel) accurately triturated and mixed with little backfat cubes, salt, sodium nitrate and nitrite, spices and peppercorns, and then cooked in oven for many hours. The cooked ham is obtained
G. Vonghia, V. Liuzzi, M. Faccia, G. Alviti, G. Cacace, A. Di Luccia   +5 more
doaj   +1 more source

Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis [PDF]

, 2008
Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial ...
A. Gorg, C. Tastet, D. Mokranjac, F. Gharahdaghi, F. Granier, H. Zischka, J. C. Sanchez, M. Chevallet, M. Eschenbruch, P. Lescuyer, S. Iwata, S. Luche, S. Luche, S. Richert, T. Rabilloud, T. Tsukihara, V. Santoni   +16 more
core   +6 more sources

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Comparative analysis of the venom proteome of four important Malaysian snake species

Journal of Venomous Animals and Toxins including Tropical Diseases, 2014
BackgroundNaja kaouthia, Ophiophagus hannah, Bungarus fasciatusandCalloselasma rhodostomaare four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and ...
Jaya Vejayan, Too Lay Khoon, Halijah Ibrahim   +2 more
doaj   +1 more source