Results 61 to 70 of about 212,059 (288)
Isoelectric Point Separations of Peptides and Proteins
Proteomes, 2017 The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection.
Melissa R. Pergande +1 more
doaj +1 more source
Two-Dimensional Gel Electrophoresis and the Beginning of Proteomics [PDF]
Clinical Chemistry, 2014 Featured article: O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975;250:4007–21.2 No one had heard of proteomics in 1971. The invention of the word was 25 years away (1). At the time, doing molecular biology meant studying bacteria or their viruses, the bacteriophage.
openaire +2 more sources
Advanced Science, EarlyView.Malectin alleviates high glucose‐induced ER stress and damage in placental trophoblasts, a function dependent on its six critical carbohydrate‐binding residues. In a GDM mouse model, administration of TAT‐Malectin ameliorated hyperglycemia and placental ER stress and prevented fetal macrosomia.
Jiahui Zhu +12 more
wiley +1 more source
Advanced Science, EarlyView.Phospholipid transfer protein(PLTP) plays a critical role in forming a complex with kinase A (AURKA) and P65. This interaction facilitates phosphorylation of P65 at Ser536, leading to the activation of the NF‐κB signaling pathway. Ultimately, this leads to the upregulation of downstream cytokines, including IL‐6, IL‐8, and CSF‐1, which promotes M2 ...
Xinyue Liang +14 more
wiley +1 more source
Analytical performance of a sandwich enzyme immunoassay for preβ1-HDL in stabilized plasma
Journal of Lipid Research, 2003 We have established an immunoassay for preβ1-HDL (the initial acceptor of cellular cholesterol) using a monoclonal antibody, MAb55201. Because preβ1-HDL is unstable during storage, fresh plasma must be used for preβ1-HDL measurements.
Takashi Miida +6 more
doaj +1 more source
Unraveling the Molecular Mechanisms Underlying Spontaneous Multipolar Mitosis Through CIN‐seq
Advanced Science, EarlyView.Multipolar mitosis, a hallmark of chromosomal instability (CIN), drives tumor heterogeneity but is challenging to study in live cells. Using CIN‐seq, a single‐cell multiomics method, we profiled rare CIN events and identified mechanisms associated with viable multipolar mitosis, including PTEN attenuation, Rho GTPase‐driven cytokinesis failure, and ...
Pin‐Rui Su +10 more
wiley +1 more source
مجله دانشکده پزشکی اصفهان, 2018 مقدمه: با وجود استفادهی گسترده، پوشش پروتئینی پایین و الگوهای اسمیری، از مهمترین موانع بازدارنده برای استفاده از مطالعات پروتئومیک مبتنی بر ژل در بررسیهای بالینی میباشد.
Hamidreza Abbasi +4 more
doaj +1 more source
Advanced Science, EarlyView.ABSTRACT Gut microbiota dysbiosis promotes colorectal cancer (CRC) tumorigenesis. A global fecal metagenomic analysis identified Gemella morbillorum as a key contributor to the CRC‐associated microbiota. Fluorescence in situ hybridization revealed that Gemella morbillorum is enriched in CRC tumor tissues compared to adjacent normal tissues.
Zhen Wang +8 more
wiley +1 more source
Advanced Science, EarlyView.TRF enriches Ruminococcus, a bacterial genus producing SCFAs, and activates the epithelial HIF‐1α signaling pathway. This mechanism protects the colonic mucosa from inflammatory insults in colitis models. Mechanistically, gut lactate production during starvation and refeeding mediates H4K12la, which increases SLC9A3 expression and creates an acidic gut
Linwen Huang +17 more
wiley +1 more source
Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry
Biological Procedures Online, 2010 The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass ...
Honoré Bent +4 more
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