Results 11 to 20 of about 212,059 (288)

A Novel Spot-Enhancement Anisotropic Diffusion Method for the Improvement of Segmentation in Two-dimensional Gel Electrophoresis Images, Based on the Watershed Transform Algorithm [PDF]

open access: yesIranian Journal of Medical Physics, 2015
Introduction Two-dimensional gel electrophoresis (2DGE) is a powerful technique in proteomics for protein separation. In this technique, spot segmentation is an essential stage, which can be challenging due to problems such as overlapping spots, streaks,
Sina Shamekhi   +3 more
doaj   +1 more source

Two-dimensional gel electrophoresis in proteomics: Past, present and future. [PDF]

open access: yesJournal of Proteomics, 2010
Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from
T. Rabilloud   +3 more
semanticscholar   +1 more source

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives

open access: yesBioTechniques, 2008
The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj   +1 more source

Mapa del proteoma de parásitos de Leishmania Viannia a partir de electroforesis de geles de poliacramida en dos dimensiones y otras técnicas asociadas

open access: yesBiomédica: revista del Instituto Nacional de Salud, 2003
En este estudio demostramos el potencial de la electroforesis en dos dimensiones (2DE) como herramienta para la caracterización del proteoma de Leishmania (expresión proteica complementaria del genoma).
Rafael Góngora   +5 more
doaj   +1 more source

High resolution two-dimensional electrophoresis of proteins.

open access: yesJournal of Biological Chemistry, 1975
A technique has been developed for the separation of proteins by two-dimensional polyacrylamide gel electrophoresis. Due to its resolution and sensitivity, this technique is a powerful tool for the analysis and detection of proteins from complex ...
P. O’Farrell
semanticscholar   +1 more source

Protein heterogeneity of lipoprotein particles containing apolipoprotein A-I without apolipoprotein A-II and apolipoprotein A-I with apolipoprotein A-II isolated from human plasma.

open access: yesJournal of Lipid Research, 1988
The protein heterogeneity of fractions isolated by immunoaffinity chromatography on anti-apolipoprotein A-I and anti-apolipoprotein A-II affinity columns was analyzed by high resolution two-dimensional gel electrophoresis.
R W James   +8 more
doaj   +1 more source

Comparative Two-Dimensional Fluorescence Gel Electrophoresis [PDF]

open access: yes, 2017
Abstract Comparative two-dimensional gel electrophoresis (CoFGE) is a special version of two-dimensional polyacrylamide GE (2D-PAGE) and related to difference GE (2D-DIGE). It provides reproducibility and standardisation for 2D-PAGE by introducing a reference to the experiment.
Simone König, Doreen Ackermann
openaire   +4 more sources

Proteome analysis in plant stress research: a review

open access: yesCzech Journal of Genetics and Plant Breeding, 2007
Proteomic techniques that allow the identification and quantification of stress-related proteins, mapping of dynamics of their expression and posttranslational modifications represent an important approach in the research of plant stresses.
Pavel Vítámvás   +2 more
doaj   +1 more source

Hierarchical transformation-guided image registration for two-dimensional gel electrophoresis image

open access: yesJournal of Algorithms & Computational Technology, 2020
Many proteomics studies have been devoted to investigate the gel-based methods for separation and fractionation of complex protein mixture extracted from tissues, cells, and other biological specimens.
Lifang Wei   +5 more
doaj   +1 more source

Identification of Bacterial Proteins in the Gut of Sunn pest, Eurygaster integriceps [PDF]

open access: yesJournal of Cell and Molecular Research, 2021
Proteomics is a powerful tool to identify effective proteins in the biochemical reactions of the insect body. Many proteins were reported in the gut lumen and tissues which are essential to complete the physiological role of the alimentary canal of Sunn ...
Mohammad Saadati, Mahmoud Toorchi
doaj   +1 more source

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