Analysis of proteins from marine molluscs [PDF]
, 2009 Application of the two-dimensional gel electrophoresis (2DE) protocols which were developed for samples of mammalian origin gives unsatisfactory results when used in samples from marine molluscs. This chapter describes a detailed protocol of 2DE that can
Bebianno, Maria João +2 more
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Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]
, 2006 Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C +3 more
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Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives
BioTechniques, 2008 The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj +1 more source
Establishment of two-dimensional gel electrophoresis for soybean protein isolate and its application
Grain & Oil Science and Technology, 2020 To optimize the conditions for the establishment of two-dimensional gel electrophoresis (2-DE) of soybean protein isolate (SPI), we investigated Ampholine mixture, anodic and cathodic electrolytes, loading amount of sample, acrylamide concentration, pH ...
Xinkai Lu +8 more
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Comparative Two-Dimensional Fluorescence Gel Electrophoresis [PDF]
, 2017 Abstract Comparative two-dimensional gel electrophoresis (CoFGE) is a special version of two-dimensional polyacrylamide GE (2D-PAGE) and related to difference GE (2D-DIGE). It provides reproducibility and standardisation for 2D-PAGE by introducing a reference to the experiment.
Simone König, Doreen Ackermann
openaire +4 more sources
Biomédica: revista del Instituto Nacional de Salud, 2003 En este estudio demostramos el potencial de la electroforesis en dos dimensiones (2DE) como herramienta para la caracterización del proteoma de Leishmania (expresión proteica complementaria del genoma).
Rafael Góngora +5 more
doaj +1 more source
Proteome analysis in plant stress research: a review
Czech Journal of Genetics and Plant Breeding, 2007 Proteomic techniques that allow the identification and quantification of stress-related proteins, mapping of dynamics of their expression and posttranslational modifications represent an important approach in the research of plant stresses.
Pavel Vítámvás +2 more
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A robust high-sensitivity algorithm for automated detection of proteins in two-dimensional electrophoresis gels [PDF]
, 1993 The automated interpretation of two-dimensional gel electrophoresis images used in protein separation and analysis presents a formidable problem in the detection and characterization of ill-defined spatial objects.
Harrington, Michael G. +1 more
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Hierarchical transformation-guided image registration for two-dimensional gel electrophoresis image
Journal of Algorithms & Computational Technology, 2020 Many proteomics studies have been devoted to investigate the gel-based methods for separation and fractionation of complex protein mixture extracted from tissues, cells, and other biological specimens.
Lifang Wei +5 more
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Mapping cells and sub-cellular organelles on 2-D gels: ‘new tricks for an old horse’ [PDF]
, 1995 Nowadays, investigators in all fields are faced with the identification of unknown, up- or down-regulated, modified proteins that they are trying to identify.
Dahlbäck, Björn, +6 more
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