Results 231 to 240 of about 212,059 (288)

Two-Dimensional Difference Gel Electrophoresis

2009
The introduction of two-dimensional fluorescent difference gel electrophoresis has enabled the extensive screening of differential protein expression levels with higher confidence and greater sensitivity than using the classical two-dimensional electrophoresis (2DE) approach.
openaire   +2 more sources

Detection of dystrophin on two-dimensional gel electrophoresis

Biochemical and Biophysical Research Communications, 1989
A protein with MW approximately 350 k daltons and pI approximately 5.5, which was deleted in the dystrophic mouse (C57BL/10ScSn-mdx), was detected on two-dimensional gel electrophoresis with silver staining. Deletion of this protein was uniformly observed in the dystrophic mouse extensor digitus longus, soleus and cardiac muscle.
S, Hori, H, Sugiura, T, Shimizu, T, Hirabayashi, S, Ohtani, M, Yoshida, K, Miyamoto, H, Tanabe   +7 more
openaire   +2 more sources

Dialysis‐assisted two‐dimensional gel electrophoresis

ELECTROPHORESIS, 2006
Abstract2‐DE is an important tool in proteomics research. However, intrinsic gel‐to‐gel variability of 2‐DE often masks the biological differences between the samples and compromises quantitative comparison of protein expression levels. Here, we describe a modification of 2‐DE that results in improved matching and quantification of proteins.
Olivier, Danos, Fedor, Svinartchouk
openaire   +2 more sources

Two-Dimensional Gel Electrophoresis: A Reference Protocol

2017
Two-dimensional gel electrophoresis (2DE) has been a mainstay of proteomic techniques for more than four decades. It was even in use for several years before the term proteomics was actually coined in the early 1990s. Over this time, it has been used in the study of many diseases including cancer, diabetes, heart disease, and psychiatric disorders ...
Veronica M, Saia-Cereda, Adriano, Aquino, Paul C, Guest, Daniel, Martins-de-Souza   +3 more
openaire   +2 more sources

Two-dimensional gel electrophoresis

1998
Abstract Two-dimensional gel electrophoresis (2DE) has been and remains a unique and powerful approach to resolve proteins and polypeptides in complex protein mixtures. However, in the past this approach has caused as much frustration as satisfaction to investigators because of its complexity, the difficulty of achieving good ...
openaire   +1 more source

Automatic segmentation and modelling of two-dimensional electrophoresis gels

Proceedings of 3rd IEEE International Conference on Image Processing, 2002
An important issue in the analysis of two-dimensional electrophoresis images is the detection and quantification of protein spots. In this paper we describe a new robust technique to segment and model the different spots present in the gels. For the segmentation a watershed technique is applied.
Eva Bettens, Paul Scheunders, Jan Sijbers, Dirk Van Dyck, L. Moens   +4 more
openaire   +2 more sources

Two-Dimensional Gel Electrophoresis of Total Yeast Proteins

2005
Two-dimensional gel electrophoresis (2-DE) offers the opportunity of separating several hundred proteins from a total yeast cellular extract. A detailed description is provided here of the different steps required for the separation and visualization of radiolabeled yeast proteins on high-resolution (24 cm x 20 cm) 2-D gels.
Boucherie, H., Monribot-Espagne, C.
openaire   +3 more sources

In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis.

Analytical Biochemistry, 1992
J. Rosenfeld, J. Capdevielle, J. Guillemot, P. Ferrara   +3 more
semanticscholar   +1 more source

Two-dimensional agarose gel electrophoresis without gel manipulation

Analytical Biochemistry, 1985
The apparatus and procedure to perform two-dimensional agarose gel electrophoresis without manipulating the gel used for the first electrophoresis (first-dimension gel) have been developed. The procedure is less complex, less damaging to first-dimension gels, and more precise than procedures that require manipulation of the first-dimension gel.
openaire   +2 more sources

Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria

2009
Two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry (MS) is the classic proteomics approach used to monitor the dynamics of protein abundance and posttranslational modifications in biological systems. In this chapter, we provide detailed protocols for 2-DE-based proteomics of mycobacteria.
Mattow, J., Siejak, F., Hagens, K., Kreuzeder, J., Kaufmann, S., Schaible, U.   +5 more
openaire   +3 more sources