Results 211 to 220 of about 212,059 (288)
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Two‐Dimensional Gel Electrophoresis
Current Protocols in Protein Science, 1999AbstractTwo‐dimensional gel electrophoresis combines two different electrophoretic separating techniques in perpendicular directions to provide a much greater separation of complex protein mixtures than either of the individual procedures. Variations of the most common two‐dimensional technique are described in this unit, namely isoelectrofocusing (IEF)
S. Harper, J. Mozdzanowski, D. Speicher
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Two-dimensional gel electrophoresis of membrane proteins.
Biochemistry, 1976A high-resolution method for two-dimensional separation of membrane proteins is described. It involves a nondiscriminating solubilization of a membrane preparation with sodium dodecyl sulfate, followed by electrophoresis in the first dimension according to charge (by isoelectric focusing).
G. Ames, K. Nikaido
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Two-Dimensional Gel Electrophoresis and 2D-DIGE.
Methods in molecular biology, 2018Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to be one of the most versatile and widely used techniques to study the proteome of a biological system. In particular, a modified version of 2D-PAGE, two-dimensional difference gel electrophoresis (2D-DIGE), which uses differential labeling of protein samples with up to three ...
P. Meleady
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Sample preparation for two‐dimensional gel electrophoresis
PROTEOMICS, 2003Abstract The choice of sample preparation protocol is a critical influential factor for isoelectric focusing which in turn affects the two‐dimensional gel result in terms of quality and protein species distribution. The optimal protocol varies depending on the nature of the sample for analysis and the properties of the constituent ...
Margaret Shaw, B. Riederer
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Preprocessing of two‐dimensional gel electrophoresis images
PROTEOMICS, 2004Abstract Proteomics produces a huge amount of two‐dimensional gel electrophoresis images. Their analysis can yield a lot of information concerning proteins responsible for different diseases or new unidentified proteins. However, an automatic analysis of such images requires an efficient tool for reducing noise in
K. Kaczmarek +3 more
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Two-dimensional gel electrophoresis in the light of new developments
TrAC - Trends in Analytical Chemistry, 2014Paweł Pomastowski +1 more
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Two‐Dimensional Gel Electrophoresis
Current Protocols in Immunology, 2004AbstractTwo‐dimensional gel electrophoresis is the combination of two high‐resolution electrophoretic procedures (isoelectric focusing and SDS‐polyacrylamide gel electrophoresis) to provide much greater resolution than either procedure alone. In the first‐dimension gel, solubilized proteins are separated according to their isoelectric point (pI) by ...
Lonnie D, Adams, Sean R, Gallagher
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Two-Dimensional Difference Gel Electrophoresis
Nature Protocols, 2006The two-dimensional (2D) polyacrylamide gel-based approach to protein profiling has been successful because it is an accessible, inexpensive, and powerful tool for the analysis of global patterns of protein expression. All protein spots that are resolved and detected within the 104 to 105 dynamic range of gel capacity can be studied qualitatively and ...
Surya, Viswanathan +2 more
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