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Two-photon excitation microscopy
2003Publisher Summary The chapter discusses the basic principle, architecture, and applications of two-photon excitation (TPE) microscopy. Fluorescence microscopy is a very popular contrast mechanism for imaging in biology as fluorescence is highly specific either as exogenous labeling or endogenous autofluorescence.
DIASPRO, ALBERTO GIOVANNI, CHIRICO G.
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Two-photon Microscopy and Imaging
2006Multiphoton microscopy (MPM) is an imaging technique that employs signals, such as fluorescence, generated by a multiphoton absorption (MPA) process as its contrast mechanism. Compared to other fluorescence microscopy techniques, MPM often has a higher sensitivity, superior penetration into scattering tissue, and causes less photodamage to the sample ...
Patrick Theer +3 more
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Imaging of Rhodopsin Crystals with Two-Photon Microscopy
2015Two-photon microscopy has been shown to be an invaluable tool for detecting and monitoring protein crystallization trials and characterizing membrane protein crystals. This imaging method has proven especially useful for rhodopsin, because of the dependence of rhodopsin's fluorescence spectra on the isomerization state of its intrinsic chromophore ...
Grazyna, Palczewska, David, Salom
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Abstract This chapter discusses the phenomenon of two-photon (2P) absorption and fluorescence emission. Included is a thorough discussion of the Göppert Mayer phenomenon, which is the absorption under extraordinary circumstances of two infrared (IR) photons with the emission of a single visible wavelength photon.
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Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy
Cell, 2022Maria M Korympidou +2 more
exaly
Two-Photon-Excited Photoinduced Force Microscopy
Nano LettersSensing photoexcitation-induced forces via atomic force microscopy (AFM) has attracted much attention as a promising nanoscale molecular imaging modality. However, most such nanospectroscopies suffer from linear background and noise under single-photon excitation, including scattering into a quadrant detector or photothermal response from the ...
Tetsu Tamura +5 more
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Introduction to two‐photon microscopy
Microscopy Research and Technique, 1999openaire +3 more sources