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Two-Photon Fluorescence Microscopy of Cerebral Hemodynamics
Cold Spring Harbor Protocols, 2010INTRODUCTIONUnder physiological conditions, neuronal activity is tightly coupled to hemodynamics of the surrounding microvessels. Conversely, most brain diseases are associated with a disturbance in neurovascular coupling. Measuring the hemodynamic response of the microvascular network to neuronal stimulation in vivo involves two major challenges ...
Liis, Lindvere +2 more
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Abstract This chapter discusses the phenomenon of two-photon (2P) absorption and fluorescence emission. Included is a thorough discussion of the Göppert Mayer phenomenon, which is the absorption under extraordinary circumstances of two infrared (IR) photons with the emission of a single visible wavelength photon.
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Two‐photon fluorescence surface wave microscopy
Journal of Microscopy, 2005SummaryThis paper demonstrates the principle of two‐photon surface wave microscopy with a view to applications on biological samples. We describe a modified scanning optical microscope, which uses specially prepared coverslips. These coverslips are designed to support the propagation of surface waves capable of large field enhancements. We also discuss
J Y L, Goh +5 more
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Imaging of Rhodopsin Crystals with Two-Photon Microscopy
2015Two-photon microscopy has been shown to be an invaluable tool for detecting and monitoring protein crystallization trials and characterizing membrane protein crystals. This imaging method has proven especially useful for rhodopsin, because of the dependence of rhodopsin's fluorescence spectra on the isomerization state of its intrinsic chromophore ...
Grazyna, Palczewska, David, Salom
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Two-Photon-Excited Photoinduced Force Microscopy
Nano LettersSensing photoexcitation-induced forces via atomic force microscopy (AFM) has attracted much attention as a promising nanoscale molecular imaging modality. However, most such nanospectroscopies suffer from linear background and noise under single-photon excitation, including scattering into a quadrant detector or photothermal response from the ...
Tetsu Tamura +5 more
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Sustained Deep-Tissue Voltage Recording Using a Fast Indicator Evolved for Two-Photon Microscopy
Cell, 2022François St-Pierre +2 more
exaly
Introduction to two‐photon microscopy
Microscopy Research and Technique, 1999openaire +3 more sources