Results 261 to 270 of about 288,583 (303)

In Vivo Two-Photon Microscopy of Microglia

2013
In vivo imaging with two-photon microscopy is becoming an indispensable technique to investigate cellular and subcellular phenomenon in living tissues including the central nervous system. This microscopy enables to image dynamics of molecules, morphology, and excitability with minimal invasion to tissues. Microglia are residual immune-responsive cells
Satoru, Kondo, Shigeo, Okabe
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Two-Photon Laser Scanning Fluorescence Microscopy

Science, 1990
Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption in the ultraviolet with a stream of strongly focused subpicosecond pulses of red laser light has made possible fluorescence images of
Denk, W., Strickler, J., Webb, W.
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Two‐Photon Microscopy and Spectroscopy of Lanthanide Bioprobes

ChemPhysChem, 2007
AbstractThe linear and non‐linear photophysical properties of tris‐dipicolinate europium and terbium complexes (absorption, emission, lifetime, luminescence induced by two‐photon absorption) are studied in the crystalline state as well as in protein derivative crystals and compared to those in solution. Upon laser irradiation at 532 nm, luminescence of
d'Aléo, Anthony, Pompidor, Guillaume, Elena-Herrmann, Bénédicte, Vicat, Jean, Baldeck, Patrice L., Toupet, Loic, Kahn, Richard, Andraud, Chantal, Maury, Olivier   +8 more
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Saturation in two-photon microscopy

SPIE Proceedings, 2004
Excitation saturation and other photophysical dynamics can have a dramatic influence on the effective imaging point spread function (psf) in fluorescence microscopy. Specifically, saturation leads to increased fluorescence observation volumes and altered spatial profiles for the psf.
Gianguido C. Cianci, Keith M. Berland
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Two-Photon Microscopy for the Study of Tendons

Journal of Visualized Experiments
Two-photon microscopy has emerged as a potent tool for evaluating deep tissue cells and characterizing the alignment of the extracellular matrix (ECM) in various biological systems. This technique relies on nonlinear light-matter interactions to detect two distinct signals: the second harmonic generated (SHG) diffusion signal, which facilitates the ...
Steffany, Villaseñor, Mor, Grinstein
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Innovations in two-photon deep tissue microscopy

IEEE Engineering in Medicine and Biology Magazine, 1999
The goal of this article is to provide a review of deep tissue studies based on two-photon microscopy. We will further explore three new developments that have significantly enhanced the power of two-photon imaging: (1) simultaneous two-photon fluorescence and confocal reflected-light imaging, (2) two-photon video-rate imaging, and (3) fluorescent ...
C, Buehler, K H, Kim, C Y, Dong, B R, Masters, P T, So   +4 more
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Two‐photon fluorescence surface wave microscopy

Journal of Microscopy, 2005
SummaryThis paper demonstrates the principle of two‐photon surface wave microscopy with a view to applications on biological samples. We describe a modified scanning optical microscope, which uses specially prepared coverslips. These coverslips are designed to support the propagation of surface waves capable of large field enhancements. We also discuss
J Y L, Goh, M G, Somekh, C W, See, M C, Pitter, K A, Vere, P, O'Shea   +5 more
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Two-photon microscopy with enhanced contrast and resolution

Applied Optics, 2017
A method combining the saturation effect with the ratio concerned quadratic intensity weighted subtraction (RQIWS) algorithm for resolution and contrast enhancement in a two-photon microscopy system is presented in this paper. In the proposed method, the saturation effect is utilized to get a profile-extended solid spot and a center-shrunken doughnut ...
Shiyi, Sun, Shaocong, Liu, Cuifang, Kuang, Xu, Liu   +3 more
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Two-Photon Fluorescence Microscopy of Cerebral Hemodynamics

Cold Spring Harbor Protocols, 2010
INTRODUCTIONUnder physiological conditions, neuronal activity is tightly coupled to hemodynamics of the surrounding microvessels. Conversely, most brain diseases are associated with a disturbance in neurovascular coupling. Measuring the hemodynamic response of the microvascular network to neuronal stimulation in vivo involves two major challenges ...
Liis, Lindvere, Adrienne, Dorr, Bojana, Stefanovic   +2 more
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Effect of a confocal pinhole in two-photon microscopy

Microscopy Research and Technique, 1999
We investigated the effect of a finite-sized confocal pinhole on the performance of nonlinear optical microscopes based on two-photon excited fluorescence and second-harmonic generation. These techniques were implemented using a modified inverted commercial confocal microscope coupled to a femtosecond Ti:sapphire laser.
R, Gauderon, P B, Lukins, C J, Sheppard
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