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Dimension-based quantification of aging-associated cerebral microvasculature determined by optical coherence tomography and two-photon microscopy. [PDF]
J BiophotonicsYan F +11 more
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<i>In Vivo</i> Two-Photon Microscopy Reveals Sensory-Evoked Serotonin (5-HT) Release in Adult Mammalian Neocortex. [PDF]
ACS Chem NeurosciOcana-Santero G +3 more
europepmc +1 more source
Two-Photon Uncaging Microscopy
Cold Spring Harbor Protocols, 2011 INTRODUCTIONTwo-photon uncaging takes advantage of the inherent optical sectioning power of two-photon excitation to generate highly localized concentration increases of neurotransmitters such as glutamate. This can be used to activate isolated clusters of receptors and, thus, produce maps of receptor densities, or activate intracellular signal ...
Masanori, Matsuzaki, Haruo, Kasai
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Deep tissue two-photon microscopy
Nature Methods, 2005With few exceptions biological tissues strongly scatter light, making high-resolution deep imaging impossible for traditional-including confocal-fluorescence microscopy. Nonlinear optical microscopy, in particular two photon-excited fluorescence microscopy, has overcome this limitation, providing large depth penetration mainly because even multiply ...
Fritjof Helmchen +2 more
exaly +6 more sources
Two-Photon Microscopy with a Double-Wavelength Metasurface Objective Lens [PDF]
Nano Letters, 2018 Two-photon microscopy is a key imaging technique in life sciences due to its superior deep-tissue imaging capabilities. Light-weight and compact two-photon microscopes are of great interest because of their applications for in vivo deep brain imaging ...
Ehsan Arbabi +2 more
exaly +2 more sources
Two-Photon Phosphorescence Lifetime Microscopy
2021Two-photon Phosphorescence Lifetime Microscopy (2PLM) is an emerging nonlinear optical technique that has great potential to improve our understanding of the basic biology underlying human health and disease. Although analogous to 2-photon Fluorescence Lifetime Imaging Microscopy (2P-FLIM), the contrast in 2PLM is fundamentally different from various ...
Nastaran, Abbasizadeh, Joel A, Spencer
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Two-Photon Laser Scanning Microscopy
, 2008 Since its inception more than 15 years ago, two-photon laser scanning microscopy (2PLSM) has found widespread use in biological and medical research. Two-photon microscopy is based on simultaneous absorption of two photons by fluorophores and subsequent ...
Nimmerjahn, A, Theer, P, Helmchen, F
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2014
Two-photon (TP) laser scanning microscopy has become an important tool for noninvasive biomedical imaging. TP fluorophore excitation using (near) infrared wavelengths show enhanced tissue penetration and reduced photodamage of biological specimen. This modality allows noninvasive three-dimensional deep-tissue imaging with submicron resolution, which ...
Wu, Zhuojun, van Zandvoort, Marc
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Two-photon (TP) laser scanning microscopy has become an important tool for noninvasive biomedical imaging. TP fluorophore excitation using (near) infrared wavelengths show enhanced tissue penetration and reduced photodamage of biological specimen. This modality allows noninvasive three-dimensional deep-tissue imaging with submicron resolution, which ...
Wu, Zhuojun, van Zandvoort, Marc
openaire +1 more source
Two-photon Microscopy Of Tissues
2003Tissue physiology and pathology can be better understood using two-photon microscopy that allows tissue structure and biochemistry to be assayed on the subcellular level. Histological analysis provides high-resolution tissue characterization and is the only imaging method that can be applied to tissues of arbitrary thicknesses.
Peter T. C. So +8 more
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