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In Vivo Two-Photon Microscopy of Microglia
2013In vivo imaging with two-photon microscopy is becoming an indispensable technique to investigate cellular and subcellular phenomenon in living tissues including the central nervous system. This microscopy enables to image dynamics of molecules, morphology, and excitability with minimal invasion to tissues. Microglia are residual immune-responsive cells
Satoru, Kondo, Shigeo, Okabe
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Two‐Photon Microscopy and Spectroscopy of Lanthanide Bioprobes
ChemPhysChem, 2007AbstractThe linear and non‐linear photophysical properties of tris‐dipicolinate europium and terbium complexes (absorption, emission, lifetime, luminescence induced by two‐photon absorption) are studied in the crystalline state as well as in protein derivative crystals and compared to those in solution. Upon laser irradiation at 532 nm, luminescence of
d'Aléo, Anthony +8 more
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Two-Photon Laser Scanning Fluorescence Microscopy
Science, 1990Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption in the ultraviolet with a stream of strongly focused subpicosecond pulses of red laser light has made possible fluorescence images of
Denk, W., Strickler, J., Webb, W.
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Saturation in two-photon microscopy
SPIE Proceedings, 2004Excitation saturation and other photophysical dynamics can have a dramatic influence on the effective imaging point spread function (psf) in fluorescence microscopy. Specifically, saturation leads to increased fluorescence observation volumes and altered spatial profiles for the psf.
Gianguido C. Cianci, Keith M. Berland
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Two-Photon Microscopy for the Study of Tendons
Journal of Visualized ExperimentsTwo-photon microscopy has emerged as a potent tool for evaluating deep tissue cells and characterizing the alignment of the extracellular matrix (ECM) in various biological systems. This technique relies on nonlinear light-matter interactions to detect two distinct signals: the second harmonic generated (SHG) diffusion signal, which facilitates the ...
Steffany, Villaseñor, Mor, Grinstein
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Innovations in two-photon deep tissue microscopy
IEEE Engineering in Medicine and Biology Magazine, 1999The goal of this article is to provide a review of deep tissue studies based on two-photon microscopy. We will further explore three new developments that have significantly enhanced the power of two-photon imaging: (1) simultaneous two-photon fluorescence and confocal reflected-light imaging, (2) two-photon video-rate imaging, and (3) fluorescent ...
C, Buehler +4 more
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Two-photon microscopy with enhanced contrast and resolution
Applied Optics, 2017A method combining the saturation effect with the ratio concerned quadratic intensity weighted subtraction (RQIWS) algorithm for resolution and contrast enhancement in a two-photon microscopy system is presented in this paper. In the proposed method, the saturation effect is utilized to get a profile-extended solid spot and a center-shrunken doughnut ...
Shiyi, Sun +3 more
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Two-photon excitation microscopy
2003Publisher Summary The chapter discusses the basic principle, architecture, and applications of two-photon excitation (TPE) microscopy. Fluorescence microscopy is a very popular contrast mechanism for imaging in biology as fluorescence is highly specific either as exogenous labeling or endogenous autofluorescence.
DIASPRO, ALBERTO GIOVANNI, CHIRICO G.
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Two-photon Microscopy and Imaging
2006Multiphoton microscopy (MPM) is an imaging technique that employs signals, such as fluorescence, generated by a multiphoton absorption (MPA) process as its contrast mechanism. Compared to other fluorescence microscopy techniques, MPM often has a higher sensitivity, superior penetration into scattering tissue, and causes less photodamage to the sample ...
Patrick Theer +3 more
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Effect of a confocal pinhole in two-photon microscopy
Microscopy Research and Technique, 1999We investigated the effect of a finite-sized confocal pinhole on the performance of nonlinear optical microscopes based on two-photon excited fluorescence and second-harmonic generation. These techniques were implemented using a modified inverted commercial confocal microscope coupled to a femtosecond Ti:sapphire laser.
R, Gauderon, P B, Lukins, C J, Sheppard
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