Results 1 to 10 of about 20,982,500 (334)
Pulse-shaping-based two-photon FRET microscopy
EPJ Web of Conferences, 2013 We present quantitative pulse-shaping-based two-photon fluorescence resonance energy transfer microscopy. We tailor the spectral phase of the excitation pulses to achieve selective excitation of donor and acceptor, demonstrating the method in live cells.
Swanson J. A. +4 more
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Journal of the American Chemical Society, 2019 Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene.
Lloyd C Murfin +14 more
semanticscholar +1 more source
Improving Focal Photostimulation of Cortical Neurons with Pre-derived Wavefront Correction
Frontiers in Cellular Neuroscience, 2017 Recent progress in neuroscience to image and investigate brain function has been made possible by impressive developments in optogenetic and opto-molecular tools.
Vincent R. Daria +6 more
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Four-dimensional multi-site photolysis of caged neurotransmitters
Frontiers in Cellular Neuroscience, 2013 Neurons receive thousands of synaptic inputs that are distributed in space and time. The systematic study of how neurons process these inputs requires a technique to stimulate multiple yet highly targeted points of interest along the neuron's ...
Mary Ann eGo +7 more
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Multi-Color Two-Photon Microscopic Imaging Based on a Single-Wavelength Excitation
Biosensors, 2022 Two-photon probes with broad absorption spectra are beneficial for multi-color two-photon microscopy imaging, which is one of the most powerful tools to study the dynamic processes of living cells.
Wei Yan +6 more
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BMC Cancer, 2023 Background Gastrointestinal stromal tumor (GIST) is currently regarded as a potentially malignant tumor, and early diagnosis is the best way to improve its prognosis.
Lianhuang Li +8 more
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Primer to Voltage Imaging With ANNINE Dyes and Two-Photon Microscopy
Frontiers in Cellular Neuroscience, 2019 ANNINE-6 and ANNINE-6plus are voltage-sensitive dyes that when combined with two-photon microscopy are ideal for recording of neuronal voltages in vivo, in both bulk loaded tissue and the dendrites of single neurons. Here, we describe in detail but for a
Bernd Kuhn, Christopher J. Roome
semanticscholar +1 more source
In vivo two-photon microscopy of the human eye
Scientific Reports, 2019 Two-photon (2P) microscopy is a powerful tool for imaging and exploring label-free biological tissues at high resolution. Although this type of microscopy has been demonstrated in ex vivo ocular tissues of both humans and animal models, imaging the human
Francisco J. Ávila +3 more
semanticscholar +1 more source
Frontiers in Immunology, 2022 BackgroundResults from our recent study demonstrate that sphingosine-1-phosphate receptor 1 (S1PR1) modulation improves microvascular hemodynamics after cerebrovascular thrombosis.
Han-Dong Li +7 more
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Deep vascular imaging in wounds by two-photon fluorescence microscopy. [PDF]
PLoS ONE, 2013 Deep imaging within tissue (over 300 μm) at micrometer resolution has become possible with the advent of two-photon fluorescence microscopy (2PFM). The advantages of 2PFM have been used to interrogate endogenous and exogenous fluorophores in the skin ...
Ciceron O Yanez +6 more
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