Results 31 to 40 of about 20,982,500 (334)

Adaptive Optics in Two-Photon Microscopy and Two-Photon Lithography

open access: yes, 2018
Two-photon microscopy has become an indispensable tool in biological research allowing imaging of intracellular structures especially deep in tissue. Due to its capability of three-dimensional (3D) fabrication in lithography, two-photon excitation is also an essential tool for nanofabrication.
Neuwirth, Julia
core   +3 more sources

ADVANCED OPTICAL TECHNIQUES TO EXPLORE BRAIN STRUCTURE AND FUNCTION [PDF]

open access: yesJournal of Innovative Optical Health Sciences, 2013
Understanding brain structure and function, and the complex relationships between them, is one of the grand challenges of contemporary sciences. Thanks to their flexibility, optical techniques could be the key to explore this complex network.
L. SILVESTRI   +4 more
doaj   +1 more source

Label-free imaging immune cells and collagen in atherosclerosis with two-photon and second harmonic generation microscopy [PDF]

open access: yesJournal of Innovative Optical Health Sciences, 2016
Atherosclerosis has been recognized as a chronic inflammation disease, in which many types of cells participate in this process, including lymphocytes, macrophages, dendritic cells (DCs), mast cells, vascular smooth muscle cells (SMCs).
Chunqiang Li   +2 more
doaj   +1 more source

Simultaneous multiplane imaging with reverberation two-photon microscopy

open access: yesNature Methods, 2020
Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue.
D. Beaulieu   +4 more
semanticscholar   +1 more source

Assessment of Extramammary Paget Disease by Two-Photon Microscopy

open access: yesFrontiers in Medicine, 2022
Two-photon microscopy techniques are non-linear optical imaging methods which are gaining momentum in the investigation of fixed tissue sections, fresh tissue or even for in vivo experiments. Two-photon excited fluorescence and second harmonic generation
Radu Hristu   +7 more
doaj   +1 more source

Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

open access: yesLight: Science & Applications, 2020
In vivo fundus imaging offers non-invasive access to neuron structures and biochemical processes in the retina. However, optical aberrations of the eye degrade the imaging resolution and prevent visualization of subcellular retinal structures.
Zhongya Qin   +7 more
semanticscholar   +1 more source

Mu-net: Multi-scale U-net for two-photon microscopy image denoising and restoration

open access: yesNeural Networks, 2020
Advances in two two-photon microscopy (2PM) have made three-dimensional (3D) neural imaging of deep cortical regions possible. However, 2PM often suffers from poor image quality because of various noise factors, including blur, white noise, and photo ...
Sehyung Lee   +4 more
semanticscholar   +1 more source

Fluorescence radial fluctuation enables two-photon super-resolution microscopy

open access: yesFrontiers in Cellular Neuroscience, 2023
Despite recent improvements in microscopy, it is still difficult to apply super-resolution microscopy for deep imaging due to the deterioration of light convergence properties in thick specimens.
Motosuke Tsutsumi   +7 more
doaj   +1 more source

Suite2p: beyond 10,000 neurons with standard two-photon microscopy

open access: yesbioRxiv, 2016
Two-photon microscopy of calcium-dependent sensors has enabled unprecedented recordings from vast populations of neurons. While the sensors and microscopes have matured over several generations of development, computational methods to process the ...
Marius Pachitariu   +7 more
semanticscholar   +1 more source

Application of multiline two-photon microscopy to functional in vivo imaging [PDF]

open access: yes, 2006
Kurtz R, Fricke M, Kalb J, Tinnefeld P, Sauer M. Application of multiline two-photon microscopy to functional in vivo imaging. Journal of Neuroscience Methods.
Kalb, J   +4 more
core   +1 more source

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