Two photon excitable graphene quantum dots for structured illumination microscopy and imaging application: lysosome specificity and tissue-dependent imaging [PDF]
, 2018 Two-photon active Graphene Quantum Dots (GQDs) are obtained from extracts of the neem root. These biocompatible GQDs are found to be suitable for structured illumination microscopy. Two-photon microscopy ensured lysosome specificity of GQDs in live cells
Sreedharan, S. +16 more
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Imaging microglia surveillance during sleep-wake cycles in freely behaving mice
eLife, 2023 Microglia surveillance manifests itself as dynamic changes in cell morphology and functional remodeling. Whether and how microglia surveillance is coupled to brain state switches during natural sleep-wake cycles remains unclear. To address this question,
Xiaochun Gu +11 more
doaj +1 more source
Study of dendritic spine compartmentalization : a correlative fluorescence light microscopy-electron microscopy approach [PDF]
, 2014 Neurons communicate with each other through synapses. Most excitatory synapses contact small protrusions called dendritic spines. Spines are connected to dendrites by a very thin stalk called the “spine neck” which restricts diffusion between the spine ...
Cyprien, Vivien
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Epifluorescence collection in two-photon microscopy
Applied Optics, 2002 We present a simple model to describe epifluorescence collection in two-photon microscopy when one images in a turbid slab with an objective. Bulk and surface scattering determine the spatial and angular distributions of the outgoing fluorescence photons at the slab surface, and geometrical optics determines how efficiently the photons are collected ...
Beaurepaire, Emmanuel, Mertz, Jérôme
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Photobleaching in Two-Photon Excitation Microscopy [PDF]
Biophysical Journal, 2000 The intensity-squared dependence of two-photon excitation in laser scanning microscopy restricts excitation to the focal plane and leads to decreased photobleaching in thick samples. However, the high photon flux used in these experiments can potentially lead to higher-order photon interactions within the focal volume.
Patterson, George H., Piston, David W.
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Evaluating performance in three-dimensional fluorescence microscopy [PDF]
, 2007 In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including ...
JOHN M. MURRAY +7 more
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Important characteristics of two-photon intravital microscopy
, 2023 Multiphoton microscopy techniques find a unique niche in intravital microscopy where whole animals could be imaged up to a tissue depth of 1 mm. However, there remains critical details of the technique that remain subject of academic debate.
Wenfa Ng (9593279)
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Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy. [PDF]
, 2011 Second and Third Harmonic Generation (SHG and THG) microscopy is based on optical effects which are induced by specific inherent physical properties of a specimen.
Krombach Fritz +11 more
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, 2022 This chapter will provide an overview of two-photon microscopy from elements of the theory underpinning fluorescence phenomena to functioning principles of a two-photon microscope including step-by-step practical advice on how to conduct an experiment ...
Sancataldo G., Barrera O., Vetri V.
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Two-Photon Fluorescence Microscopy Imaging of Cellular Oxidative Stress Using Profluorescent Nitroxides [PDF]
, 2012 A range of varying chromophore nitroxide free radicals and their nonradical methoxyamine analogues were synthesized and their linear photophysical properties examined.
Fairfull-Smith, Kathryn E. +8 more
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