Results 61 to 70 of about 20,982,500 (334)

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Two-photon microscopy : sequential imaging studies in vivo [PDF]

, 2011
Microscopists have always desired to look inside various organ tissues to study structure, function and dysfunction of their cellular constituents. In the past, this has frequently required tissue extraction and histological preparation to gain access ...
Zammit, Christian, Di Giovanni, Giuseppe, Pierucci, Massimo, Muscat, Richard, Valentino, Mario   +4 more
core  

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes

Chemical Science, 2017
We have developed a new class of two-photon absorbing dyes that are far-red emitting, water-soluble, and very bright inside cells as well as in tissue. The significant autofluorescence from yellow wavelength region in tissue imaging can be addressed by ...
Y. Jun, H. Kim, Y. Reo, Ming-Chong Dai, K. Ahn   +4 more
semanticscholar   +1 more source

All-optical sampling utilising two-photon absorption in semiconductor microcavity [PDF]

, 2005
A highly-efficient optical sampling system based on Two-Photon Absorption in a semiconductor micro-cavity is presented.
Krug, T., Maguire, Paul, J., Donegan, John Francis, Folliot, Hervé, Donegan, J.F., Barry, Liam,, Bradley, A.L., Folliot, H., Lynch, M., Barry, Liam P., Lynch, Michael, Bradley, Ann Louise, Maguire, Paul,, Maguire, Paul J., Barry, Liam, P.   +14 more
core   +2 more sources

Cranial irradiation disrupts homeostatic microglial dynamic behavior

Journal of Neuroinflammation
Cranial irradiation causes cognitive deficits that are in part mediated by microglia, the resident immune cells of the brain. Microglia are highly reactive, exhibiting changes in shape and morphology depending on the function they are performing ...
Alexandra O. Strohm, Carl Johnston, Eric Hernady, Brian Marples, M. Kerry O’Banion, Ania K. Majewska   +5 more
doaj   +1 more source

Comparative Two-Photon Fluorescence Microscopy Analysis of Riboflavin Penetration in Two Different Solutions: Dextran and Hydroxypropyl Methylcellulose

Clinical Ophthalmology, 2020
Thales Antonio Abra De Paula, Fernando Betty Cresta, Milton Ruiz Alves University of São Paulo USP, Ophthalmology, Medical School, São Paulo, Brazil
Correspondence: Thales Antonio Abra De Paula 
 Rua Apeninos 800,apt 1004, São Paulo,
De Paula TAA, Cresta FB, Alves MR
doaj  

Two-Photon Excitation Fluorescence Microscopy

Annual Review of Biomedical Engineering, 2000
▪ Abstract  Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons.
So, Peter T. C., Dong, Chen Y., Masters, Barry R., Berland, Keith M.   +3 more
openaire   +3 more sources

Effect of terahertz irradiation on DNA damage repair in living cells

FEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno, Shota Yamazaki, Hiromichi Hoshina, Masahiko Harata   +3 more
wiley   +1 more source

Two-photon excitation fluorescent spectral and decay properties of retrograde neuronal tracer Fluoro-Gold

Scientific Reports, 2021
Fluoro-Gold is a fluorescent neuronal tracer suitable for targeted deep imaging of the nervous system. Widefield fluorescence microscopy enables visualization of Fluoro-Gold, but lacks depth discrimination.
Matthew Q. Miller, Iván Coto Hernández, Jenu V. Chacko, Steven Minderler, Nate Jowett   +4 more
doaj   +1 more source