Results 71 to 80 of about 20,982,500 (334)

Skin Imaging Using Ultrasound Imaging, Optical Coherence Tomography, Confocal Microscopy, and Two-Photon Microscopy in Cutaneous Oncology

open access: yesFrontiers in Medicine, 2019
With the recognition of dermoscopy as a new medical technology and its available fee assessment in Korea comes an increased interest in imaging-based dermatological diagnosis.
B. Oh, K. H. Kim, K. Chung
semanticscholar   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

open access: yesFEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer   +7 more
wiley   +1 more source

Activity-dependent oligodendrocyte calcium dynamics and their changes in Alzheimer’s disease

open access: yesFrontiers in Cellular Neuroscience, 2023
Oligodendrocytes (OCs) form myelin around axons, which is dependent on neuronal activity. This activity-dependent myelination plays a crucial role in training and learning.
Kenji Yoshida   +9 more
doaj   +1 more source

Two-photon excitation STED microscopy

open access: yesOptics Express, 2009
We report sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy achieved by merging this technique with stimulated-emission depletion (STED). We demonstrate an easy-to-implement and promising laser combination based on a short-pulse laser source for two-photon excitation and a continuous-wave (CW) laser source for resolution
Moneron, Gael, Hell, Stefan
openaire   +4 more sources

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

open access: yesFEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Intravital imaging of megakaryocytes

open access: yesPlatelets, 2020
The dynamics of platelet formation could only be investigated since the development of two-photon microscopy in combination with suitable fluorescent labeling strategies.
David Stegner, Katrin G. Heinze
doaj   +1 more source

Shedding Light on the Formation and Structure of Kombucha Biofilm Using Two-Photon Fluorescence Microscopy

open access: yesFrontiers in Microbiology, 2021
Kombucha pellicles are often used as inoculum to produce this beverage and have become a signature feature. This cellulosic biofilm produced by acetic acid bacteria (AAB) involves yeasts, which are also part of the kombucha consortia.
Thierry Tran   +7 more
doaj   +1 more source

Automatic Graph-based Modeling of Brain Microvessels Captured with Two-Photon Microscopy

open access: yesIEEE journal of biomedical and health informatics, 2018
Graph models of cerebral vasculature derived from two-photon microscopy have shown to be relevant to study brain microphysiology. Automatic graphing of these microvessels remain problematic due to the vascular network complexity and two-photon ...
Rafat R. Damseh   +6 more
semanticscholar   +1 more source

Applications of two-photon microscopy in the neurosciences

open access: yesFrontiers in Bioscience, 2005
Thanks to deep tissue penetration of infrared light (IR), two-photon laser scanning microscopy (2PLSM) has become an important tool in modern neuroscience. Examples range from the high-resolution visualization of dendritic spines in single neurons to the monitoring of intracellular Ca++ transients in small neural networks. In the present review we give
Cruz, H. G., Luescher, Christian
openaire   +5 more sources

Hydrogel platforms for 3D cardiac tissue engineering and fibrotic disease modelling

open access: yesFEBS Open Bio, EarlyView.
Hydrogel‐based cardiac tissue models are advancing our understanding of heart health and disease. By incorporating electrical conductivity, aligned structures, and tunable mechanical properties, these platforms better replicate the complex environment of native heart tissue.
Harrison Porritt   +2 more
wiley   +1 more source

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