Results 11 to 20 of about 20,982,500 (334)
Two-Photon Microscopy With a Double-Wavelength Metasurface Objective Lens
Nano Letters, 2018 Two-photon microscopy is a key imaging technique in life sciences due to its superior deep-tissue imaging capabilities. Light-weight and compact two-photon microscopes are of great interest because of their applications for in vivo deep brain imaging ...
Viviana Gradinaru, Yu Horie, Amir Arbabi
exaly +3 more sources
Sustained Deep-Tissue Voltage Recording Using a Fast Indicator Evolved for Two-Photon Microscopy
Cell, 2022 SUMMARY Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators enable a narrow range of applications due to poor performance under two-photon microscopy, a method ...
François St-Pierre +2 more
exaly +2 more sources
A general design of pyridinium-based fluorescent probes for enhancing two-photon microscopy. [PDF]
Biosens Bioelectron, 2023 Two-photon absorbing fluorescent probes have emerged as powerful imaging tools for subcellular-level monitoring of biological substances and processes, offering advantages such as deep light penetration, minimal photodamage, low autofluorescence, and ...
Chen R +12 more
europepmc +2 more sources
Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy
Photonics, 2023 We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning and background rejection are all improved over nonconfocal two-photon ...
Colin J. R. Sheppard +7 more
doaj +2 more sources
Two-photon microscopy for microrobotics: Visualization of micro-agents below fixed tissue. [PDF]
PLoS ONE, 2023 Optical microscopy is frequently used to visualize microrobotic agents (i.e., micro-agents) and physical surroundings with a relatively high spatio-temporal resolution.
Juan J Huaroto +7 more
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Two-Photon Uncaging Microscopy
Cold Spring Harbor Protocols, 2011 INTRODUCTIONTwo-photon uncaging takes advantage of the inherent optical sectioning power of two-photon excitation to generate highly localized concentration increases of neurotransmitters such as glutamate. This can be used to activate isolated clusters of receptors and, thus, produce maps of receptor densities, or activate intracellular signal ...
Masanori, Matsuzaki, Haruo, Kasai
openaire +3 more sources
Cell, 2023 Holistic understanding of physio-pathological processes requires noninvasive 3D imaging in deep tissue across multiple spatial and temporal scales to link diverse transient subcellular behaviors with long-term physiogenesis. Despite broad applications of
Dai Qionghai, Yeyi Cai, Zhifeng Zhao
exaly +2 more sources
Intravital two-photon microscopy of the native mouse thymus. [PDF]
PLoS ONEThe thymus, a key organ in the adaptive immune system, is sensitive to a variety of insults including cytotoxic preconditioning, which leads to atrophy, compression of the blood vascular system, and alterations in hemodynamics.
Negar Seyedhassantehrani +5 more
doaj +2 more sources
, 2014 Two-photon (TP) laser scanning microscopy has become an important tool for noninvasive biomedical imaging. TP fluorophore excitation using (near) infrared wavelengths show enhanced tissue penetration and reduced photodamage of biological specimen. This modality allows noninvasive three-dimensional deep-tissue imaging with submicron resolution, which ...
Wu, Zhuojun, van Zandvoort, Marc
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The Micro-Structure of the Celiac Ganglia—A Two-Photon Microscopy Study on Parkinson’s Disease [PDF]
DiagnosticsBackground/Objectives: This study explores the micro-structure of celiac ganglia using two-photon microscopy (TPM) to highlight histological features in neurodegenerative conditions.
Diana-Theodora Morgos +9 more
doaj +2 more sources