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Visualising Neutrophil Actin Dynamics in Zebrafish in Response to Laser Wounding Using Two-Photon Microscopy. [PDF]
Williantarra I +2 more
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Multibeam continuous axial scanning two-photon microscopy for in vivo volumetric imaging in mouse brain. [PDF]
Ataka M +7 more
europepmc +1 more source
Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy. [PDF]
Guo F +14 more
europepmc +1 more source
Combined flat-field and frequency filter approach to correcting artifacts of multichannel two-photon microscopy. [PDF]
Knapp T +5 more
europepmc +1 more source
Deep Tissue Two-Photon Microscopy
With few exceptions biological tissues strongly scatter light, making high-resolution deep imaging impossible for traditional-including confocal-fluorescence microscopy. Nonlinear optical microscopy, in particular two photon-excited fluorescence microscopy, has overcome this limitation, providing large depth penetration mainly because even multiply ...
Winfried Denk, Fritjof Helmchen
exaly +8 more sources
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Two-Photon Laser Scanning Fluorescence Microscopy
Science, 1990Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption in the ultraviolet with a stream of strongly focused subpicosecond pulses of red laser light has made possible fluorescence images of
Winfried Denk +2 more
exaly +5 more sources
Two-Photon Microscopy of Cells and Tissue [PDF]
Two-photon excitation fluorescence imaging provides thin optical sections from deep within thick, scattering specimens by way of restricting fluorophore excitation (and thus emission) to the focal plane of the microscope. Spatial confinement of two-photon excitation gives rise to several advantages over single-photon confocal microscopy.
Michael Rubart
exaly +3 more sources
Phase sensitive two-photon microscopy
Conference on Lasers and Electro-Optics, 2021Phase sensitive two-photon microscopy is demonstrated using transport-of-intensity equation-based phase retrieval algorithm. Amplitude and phase information of the complex field of two-photon microscopy signal has been retrieved for fluorescence bead and
N. Soni +3 more
semanticscholar +1 more source
ACS Nano, 2018
Currently, a serious problem obstructing the large-scale clinical applications of fluorescence technique is the shallow penetration depth. Two-photon fluorescence microscopic imaging with excitation in the longer-wavelength near-infrared (NIR) region ...
Ji Qi +16 more
semanticscholar +1 more source
Currently, a serious problem obstructing the large-scale clinical applications of fluorescence technique is the shallow penetration depth. Two-photon fluorescence microscopic imaging with excitation in the longer-wavelength near-infrared (NIR) region ...
Ji Qi +16 more
semanticscholar +1 more source

