Results 281 to 290 of about 20,982,500 (334)

Two-Photon Phosphorescence Lifetime Microscopy

2021
Two-photon Phosphorescence Lifetime Microscopy (2PLM) is an emerging nonlinear optical technique that has great potential to improve our understanding of the basic biology underlying human health and disease. Although analogous to 2-photon Fluorescence Lifetime Imaging Microscopy (2P-FLIM), the contrast in 2PLM is fundamentally different from various ...
Nastaran, Abbasizadeh, Joel A, Spencer
openaire   +2 more sources

Two-photon Microscopy Of Tissues

2003
Tissue physiology and pathology can be better understood using two-photon microscopy that allows tissue structure and biochemistry to be assayed on the subcellular level. Histological analysis provides high-resolution tissue characterization and is the only imaging method that can be applied to tissues of arbitrary thicknesses.
Peter T. C. So, Ki H. Kim, Lily Hsu, Peter Kaplan, Tom Hacewicz, Chen Y. Dong, Urs Greuter, Nick Schlumpf, Christof Buehler   +8 more
openaire   +1 more source

Volumetric two-photon microscopy with a non-diffracting Airy beam.

Optics Letters, 2019
We demonstrate a volumetric two-photon microscopy (TPM) using the non-diffracting Airy beam as illumination. Direct mapping of the imaging trajectory shows that the Airy beam extends the axial imaging range around six times longer than a traditional ...
Xiao-Jie Tan, Ci-Hang Kong, Yuxuan Ren, Cora S. W. Lai, K. Tsia, K. Wong   +5 more
semanticscholar   +1 more source

In Vivo Two-Photon Microscopy of Microglia

2013
In vivo imaging with two-photon microscopy is becoming an indispensable technique to investigate cellular and subcellular phenomenon in living tissues including the central nervous system. This microscopy enables to image dynamics of molecules, morphology, and excitability with minimal invasion to tissues. Microglia are residual immune-responsive cells
Satoru, Kondo, Shigeo, Okabe
openaire   +2 more sources

Two‐Photon Microscopy and Spectroscopy of Lanthanide Bioprobes

ChemPhysChem, 2007
AbstractThe linear and non‐linear photophysical properties of tris‐dipicolinate europium and terbium complexes (absorption, emission, lifetime, luminescence induced by two‐photon absorption) are studied in the crystalline state as well as in protein derivative crystals and compared to those in solution. Upon laser irradiation at 532 nm, luminescence of
d'Aléo, Anthony, Pompidor, Guillaume, Elena-Herrmann, Bénédicte, Vicat, Jean, Baldeck, Patrice L., Toupet, Loic, Kahn, Richard, Andraud, Chantal, Maury, Olivier   +8 more
openaire   +3 more sources

Saturation in two-photon microscopy

SPIE Proceedings, 2004
Excitation saturation and other photophysical dynamics can have a dramatic influence on the effective imaging point spread function (psf) in fluorescence microscopy. Specifically, saturation leads to increased fluorescence observation volumes and altered spatial profiles for the psf.
Gianguido C. Cianci, Keith M. Berland
openaire   +1 more source

Two-Photon Microscopy for the Study of Tendons

Journal of Visualized Experiments
Two-photon microscopy has emerged as a potent tool for evaluating deep tissue cells and characterizing the alignment of the extracellular matrix (ECM) in various biological systems. This technique relies on nonlinear light-matter interactions to detect two distinct signals: the second harmonic generated (SHG) diffusion signal, which facilitates the ...
Steffany, Villaseñor, Mor, Grinstein
openaire   +2 more sources

Two-color two-photon 4Pi fluorescence microscopy

Optics Letters, 2004
In 4Pi fluorescence microscopy the point-spread function is composed of a strong central lobe accompanied by interference sidelobes that produce artifacts in the image. We propose to combine two-color two-photon fluorescence microscopy and 4Pi fluorescence microscopy to overcome this sidelobe problem.
Jianfang, Chen, Katsumi, Midorikawa
openaire   +2 more sources

Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

Nature Methods, 2021
Wei-Jian Zong, Run-Long Wu, Shi-Yuan Chen, Junjie Wu, Hanbin Wang, Zhe Zhao, Guoqing Chen, Rui Tu, Dan-Lei Wu, Yan-Hui Hu, Yangyang Xu, Yao Wang, Zhuoli Duan, Haitao Wu, Yunfeng Zhang, Jue Zhang, Ai-Min Wang, Liang-Yi Chen, He-Ping Cheng   +18 more
semanticscholar   +1 more source

Innovations in two-photon deep tissue microscopy

IEEE Engineering in Medicine and Biology Magazine, 1999
The goal of this article is to provide a review of deep tissue studies based on two-photon microscopy. We will further explore three new developments that have significantly enhanced the power of two-photon imaging: (1) simultaneous two-photon fluorescence and confocal reflected-light imaging, (2) two-photon video-rate imaging, and (3) fluorescent ...
C, Buehler, K H, Kim, C Y, Dong, B R, Masters, P T, So   +4 more
openaire   +2 more sources