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Two‐photon fluorescence surface wave microscopy
Journal of Microscopy, 2005SummaryThis paper demonstrates the principle of two‐photon surface wave microscopy with a view to applications on biological samples. We describe a modified scanning optical microscope, which uses specially prepared coverslips. These coverslips are designed to support the propagation of surface waves capable of large field enhancements. We also discuss
J Y L, Goh +5 more
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Two-photon microscopy with enhanced contrast and resolution
Applied Optics, 2017A method combining the saturation effect with the ratio concerned quadratic intensity weighted subtraction (RQIWS) algorithm for resolution and contrast enhancement in a two-photon microscopy system is presented in this paper. In the proposed method, the saturation effect is utilized to get a profile-extended solid spot and a center-shrunken doughnut ...
Shiyi, Sun +3 more
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Two-photon excitation microscopy
2003Publisher Summary The chapter discusses the basic principle, architecture, and applications of two-photon excitation (TPE) microscopy. Fluorescence microscopy is a very popular contrast mechanism for imaging in biology as fluorescence is highly specific either as exogenous labeling or endogenous autofluorescence.
DIASPRO, ALBERTO GIOVANNI, CHIRICO G.
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Two-Photon Fluorescence Microscopy of Cerebral Hemodynamics
Cold Spring Harbor Protocols, 2010INTRODUCTIONUnder physiological conditions, neuronal activity is tightly coupled to hemodynamics of the surrounding microvessels. Conversely, most brain diseases are associated with a disturbance in neurovascular coupling. Measuring the hemodynamic response of the microvascular network to neuronal stimulation in vivo involves two major challenges ...
Liis, Lindvere +2 more
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Effect of a confocal pinhole in two-photon microscopy
Microscopy Research and Technique, 1999We investigated the effect of a finite-sized confocal pinhole on the performance of nonlinear optical microscopes based on two-photon excited fluorescence and second-harmonic generation. These techniques were implemented using a modified inverted commercial confocal microscope coupled to a femtosecond Ti:sapphire laser.
R, Gauderon, P B, Lukins, C J, Sheppard
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Abstract This chapter discusses the phenomenon of two-photon (2P) absorption and fluorescence emission. Included is a thorough discussion of the Göppert Mayer phenomenon, which is the absorption under extraordinary circumstances of two infrared (IR) photons with the emission of a single visible wavelength photon.
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Two-photon Microscopy and Imaging
2006Multiphoton microscopy (MPM) is an imaging technique that employs signals, such as fluorescence, generated by a multiphoton absorption (MPA) process as its contrast mechanism. Compared to other fluorescence microscopy techniques, MPM often has a higher sensitivity, superior penetration into scattering tissue, and causes less photodamage to the sample ...
Patrick Theer +3 more
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Imaging of Rhodopsin Crystals with Two-Photon Microscopy
2015Two-photon microscopy has been shown to be an invaluable tool for detecting and monitoring protein crystallization trials and characterizing membrane protein crystals. This imaging method has proven especially useful for rhodopsin, because of the dependence of rhodopsin's fluorescence spectra on the isomerization state of its intrinsic chromophore ...
Grazyna, Palczewska, David, Salom
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Fast high-resolution miniature two-photon microscopy for brain imaging in freely behaving mice
Nature Methods, 2017Wei-Jian Zong +16 more
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