Results 31 to 40 of about 63,885 (332)

Ubiquitin in Motion: Structural Studies of the Ubiquitin-Conjugating Enzyme∼Ubiquitin Conjugate [PDF]

Biochemistry, 2011
Ubiquitination of proteins provides a powerful and versatile post-translational signal in the eukaryotic cell. The formation of a thioester bond between ubiquitin (Ub) and the active site of a ubiquitin-conjugating enzyme (E2) is critical for the transfer of Ub to substrates. Assembly of a functional ubiquitin ligase (E3) complex poised for Ub transfer
Rachel E. Klevit, Jonathan N. Pruneda, Peter S. Brzovic, Kate E. Stoll, Laura Bolton   +4 more
openaire   +3 more sources

Solution Structure of the Ubiquitin-conjugating Enzyme UbcH5B [PDF]

Journal of Molecular Biology, 2004
The ubiquitination pathway is the main pathway for protein degradation in eukaryotic cells. The attachment of ubiquitin to a substrate protein is catalyzed by three types of enzymes, namely a ubiquitin activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin ligase (E3). Here, the structure of the human ubiquitin-conjugating enzyme (
Houben, K, Dominguez, C, van Schaik FM, Timmers, HT, Bonvin, AM, Boelens, R   +5 more
openaire   +6 more sources

Determinants of Functionality in the Ubiquitin Conjugating Enzyme Family [PDF]

Structure, 2004
The E2 enzymes are key enzymes in the ubiquitin and ubiquitin-like protein ligation pathways. To understand the functionality of the different E2 enzymes, we analyzed 190 protein sequences and 211 structures and electrostatic potentials. Key findings include: The ScUbc1 orthologs are defined by a C-terminal UBA domain. An N-terminal sequence motif that
Peter J. Winn, Amit Banerjee, James N.D. Battey, Tomasz L. Religa, Rebecca C. Wade   +4 more
openaire   +3 more sources

Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades

BMC Molecular and Cell Biology, 2020
Background Ubiquitination and ubiquitin-like protein post-translational modifications play an enormous number of roles in cellular processes. These modifications are constituted of multistep reaction cascades.
Gabriel Fenteany, Paras Gaur, Gaurav Sharma, Lajos Pintér, Ernő Kiss, Lajos Haracska   +5 more
doaj   +1 more source

NEDD8-conjugating enzyme E2s: critical targets for cancer therapy

Cell Death Discovery, 2023
NEDD8-conjugating enzymes, E2s, include the well-studied ubiquitin-conjugating enzyme E2 M (UBE2M) and the poorly characterized ubiquitin-conjugating enzyme E2 F (UBE2F).
Lisha Zhou, Xiongzhi Lin, Jin Zhu, Luyi Zhang, Siyuan Chen, Hui Yang, Lijun Jia, Baofu Chen   +7 more
doaj   +1 more source

UBE2C (ubiquitin-conjugating enzyme E2C) [PDF]

Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2011
Review on UBE2C (ubiquitin-conjugating enzyme E2C), with data on DNA, on the protein encoded, and where the gene is implicated.
Pallate, P, Berlingieri, MT, Fusco, A
openaire   +3 more sources

In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay

Bio-Protocol, 2022
Ubiquitination is a post-translational modification conserved across eukaryotic species. It contributes to a variety of regulatory pathways, including proteasomal degradation, DNA repair, and cellular differentiation.
Rebecca Burge, Katie Jameson, Anthony Wilkinson, Jeremy Mottram   +3 more
doaj   +1 more source

Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination [PDF]

Structure, 2019
Ubiquitin-conjugating enzymes (E2s) govern key aspects of ubiquitin signaling. Emerging evidence suggests that the activities of E2s are modulated by posttranslational modifications; the structural underpinnings, however, are largely unclear. Here, we unravel the structural basis and mechanistic consequences of a conserved autoubiquitination event near
Henning Urlaub, Henning Urlaub, Christoph A. Sotriffer, Mathias Diebold, Lu Yu, Theodoros I. Roumeliotis, Alena Kucerova, Jörg Mansfeld, Kristian Schweimer, Jyoti S. Choudhary, Sonja Lorenz, Olexandr Dybkov, Anna K. L. Liess   +12 more
openaire   +6 more sources

Rice ubiquitin‐conjugating enzyme OsUBC26 is essential for immunity to the blast fungus Magnaporthe oryzae

Molecular plant pathology, 2021
The functions of ubiquitin‐conjugating enzymes (E2) in plant immunity are not well understood. In this study, OsUBC26, a rice ubiquitin‐conjugating enzyme, was characterized in the defence against Magnaporthe oryzae. The expression of OsUBC26 was induced
Xin Liu, Linlin Song, Heng Zhang, Yijuan Lin, Xiaolei Shen, Jiayuan Guo, Meiling Su, Gaosheng Shi, Zonghua Wang, Guo-dong Lu   +9 more
semanticscholar   +1 more source

MALDI-TOF Mass Spectrometry for interrogating ubiquitin enzymes

Frontiers in Molecular Biosciences, 2023
The attachment of ubiquitin to a substrate (ubiquitination or ubiquitylation) impacts its lifetime and regulates its function within the cell. Several classes of enzymes oversee the attachment of ubiquitin to the substrate: an E1 activating enzyme that ...
Virginia De Cesare
doaj   +1 more source