Results 101 to 110 of about 62,421 (311)

Regulation of human papillomavirus E6 oncoprotein function via a novel ubiquitin ligase FBXO4

mBio
Previous studies have shown that E6 interacts with the E6-associated protein (E6AP) ubiquitin-protein ligase and directs its ubiquitylation activity toward several specific cellular proteins, one of the most important of which is p53. Interestingly, E6AP
Arushi Vats, Luca Braga, Nezka Kavcic, Paola Massimi, Edoardo Schneider, Mauro Giacca, Laimonis A. Laimins, Lawrence Banks   +7 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase [PDF]

, 2014
Background: FAF1 is a ubiquitin-binding adaptor for the p97 ATPase and belongs to the UBA-UBX family of p97 cofactors. p97 converts the energy derived from ATP hydrolysis into conformational changes of the p97 hexamer, which allows the dissociation of ...
Tyagi, Kshitiz, Gabriela Alexandru, Melanie Wightman, Johnson, Clare, Alexandru, Gabriela, Patrick G Pedrioli, Pedrioli, Patrick G., Wood, Nicola T., Clare Johnson, Nicola T Wood, Baron, Yorann, Daniel Fountaine, Wightman, Melanie, Fountaine, Daniel, Kshitiz Tyagi, Yorann Baron   +15 more
core   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Cullin-RING Ubiquitin Ligases in Neurodevelopment and Neurodevelopmental Disorders

Biomedicines
Ubiquitination is a dynamic and tightly regulated post-translational modification essential for modulating protein stability, trafficking, and function to preserve cellular homeostasis.
Honoka Ashitomi, Tadashi Nakagawa, Makiko Nakagawa, Toru Hosoi   +3 more
doaj   +1 more source

Ubiquitination Assay for Mammalian Cells

Bio-Protocol, 2016
Ubiquitin is an 8.5 kDa protein that can be activated and conjugated by ubiquitin-activating enzyme E1 and ubiquitin-conjugating enzyme E2, respectively.
Yang Peng, Edward Wang, Guang Peng, Shiaw-Yih Lin   +3 more
doaj   +1 more source

The Role of NEDD4 E3 Ubiquitin-Protein Ligases in Parkinson's Disease. [PDF]

Genes (Basel), 2022
Conway JA, Kinsman G, Kramer ER.
europepmc   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Functional dissection of the C-terminal part of the transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator alpha (PGC-1[alpha]) [PDF]

, 2007
The superfamily of nuclear receptors is a class of transcriptional regulators that includes the receptors for steroid hormones, thyroid hormones, retinoids and vitamin D.
Emter, Roger
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source