Results 81 to 90 of about 191,822 (309)

Probing protein ubiquitination in live cells [PDF]

, 2022
The reversible attachment of ubiquitin governs the interaction, activity and degradation of proteins whereby the type and target of this conjugation determine the biological response.
Imhof, Axel, Qin, Weihua, Cardoso, M. Cristina, Steinek, Clemens, Kolobynina, Ksenia, Leonhardt, Heinrich, Cardoso, M Cristina, Forné, Ignasi   +7 more
core   +1 more source

Deciphering non-canonical ubiquitin signaling: biology and methodology

Frontiers in Molecular Biosciences
Ubiquitination is a dynamic post-translational modification that regulates virtually all cellular processes by modulating function, localization, interactions and turnover of thousands of substrates.
Nila K. van Overbeek, Tim Aguirre, Gerbrand J. van der Heden van Noort, Blagoy Blagoev, Alfred C. O. Vertegaal   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Mastermind-Like 1 Is Ubiquitinated: Functional Consequences for Notch Signaling.

PLoS ONE, 2015
Early studies demonstrated the involvement of ubiquitination of the Notch intracellular domain for rapid turnover of the transcriptional complex at Notch target genes. It was shown that this ubiquitination was promoted by the co-activator Mastermind like
Mozhgan Farshbaf, Mikael J Lindberg, Anh Truong, Zachery Bevens, Elaina Chambers, Angeliki Pournara, Annika E Wallberg, J Brandon White   +7 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Ubiquitination: Friend and foe in cancer [PDF]

, 2018
Dynamic modulation and posttranslational modification of proteins are tightly controlled biological processes that occur in response to physiological cues.
Mansour, M.A.
core   +1 more source

Research Progress on the Mechanism for the Effect of Ubiquitination on Meat Quality [PDF]

Shipin Kexue
Meat quality is closely related to consumer experience and the benefits of deep processing enterprises, and it is influenced by diverse and complex factors.
WANG Mengxue, LIU Jitong, LI Jiqiang, ZHANG Lei, LIU Chenyue, ZHAO Wenyue, CHENG Haijian, TONG Lin, MAO Yanwei, ZHANG Yimin
doaj   +1 more source

Regulation and effects of IRF-1 and p53 ubiquitination [PDF]

, 2013
Protein ubiquitination is a key regulator of both protein stability and activity, and is involved in the regulation of a vast variety of cellular pathways.
Landré, Vivien
core  

Ubiquitination in osteosarcoma: unveiling the impact on cell biology and therapeutic strategies

Cancer Biology & Medicine
Ubiquitination, a multifaceted post-translational modification, regulates protein function, degradation, and gene expression. The pivotal role of ubiquitination in the pathogenesis and progression of cancer, including colorectal, breast, and liver cancer,
Jianlin Shen, Yue Lai, Yanjiao Wu, Xuan Lin, Cheng Zhang, Huan Liu   +5 more
doaj   +1 more source

Agnuside Stabilizes the Complex I Assembly Factor NDUFAF6 to Reinforce Mitochondrial Efficiency and Thermogenic Responsiveness

Advanced Science, EarlyView.
ABSTRACT Brown and beige adipocytes dissipate energy as heat, yet effective strategies to enhance their mitochondrial efficiency remain limited. Here, we identify Agnuside (AGN) as a selective stabilizer of the complex I assembly factor NDUFAF6. AGN directly binds cytosolic NDUFAF6, suppresses its ubiquitination, prolongs its half‐life, and facilitates
Qingwen Zhao, Li Xie, Qianzhuo Wang, Shuying Dai, Bei Li, Yingjuan Zhang, Zhe Sun, Yue Gao   +7 more
wiley   +1 more source