Results 41 to 50 of about 25,165 (253)
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
Improving parameter priors for data-scarce estimation problems
, 2013 Runoff prediction in ungauged catchments is a recurrent problem in hydrology. Conceptual models are usually calibrated by defining a feasible parameter range and then conditioning parameter sets on observed system responses, e.g., streamflow. In ungauged
Buytaert, Wouter +4 more
core +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley +1 more source
Cyber Threat Intelligence Exchange [PDF]
, 2020 The processing and exchange of Cyber Threat Intelligence (CTI) has become an increas- ingly important topic in recent years. This trend can be attributed to various factors.
Menges, Florian
core +1 more source
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Current problems of regulating civic participation in ensuring public safety
Муниципалитет: экономика и управлениеIntroduction. The relevance of the research topic is due to the need to identify problems that hinder the integrated development of civic participation (including volunteers) in ensuring public safety, as well as a mechanism for their solution.
Aleksey M. Voronov, Artem V. Mazein
doaj +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
, 2014 A dieta é considerada um dos fatores que mais influencia o comportamento espacial dos primatas. Muitas pesquisas que abordam essa relação apresentam similaridades nos seus delineamentos, mas não existe uma metodologia padrão comum.
Fernández-Bolaños, Marcelo +1 more
core +1 more source