Results 61 to 70 of about 5,558,106 (237)
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Encyclopaedic dictionary on archaeology of Tatarstan:conceptual problems
Поволжская археология, 2015 Theoretical and methodological problems of creation the glossary for the preparation of encyclopedic dictionary, which is related to the Republic of Tatarstan archaeology are considered in this article.
Abdullin Khalim M. +3 more
doaj
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
Current problems of regulating civic participation in ensuring public safety
Муниципалитет: экономика и управлениеIntroduction. The relevance of the research topic is due to the need to identify problems that hinder the integrated development of civic participation (including volunteers) in ensuring public safety, as well as a mechanism for their solution.
Aleksey M. Voronov, Artem V. Mazein
doaj +1 more source
FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source
Weakly uniform rank two vector bundles on multiprojective spaces [PDF]
, 2011 Here we classify the weakly uniform rank two vector bundles on multiprojective spaces. Moreover, we show that every rank r>2 weakly uniform vector bundle with splitting type a1,1=⋯=ar,s=0 is trivial and every rank r>2 uniform vector bundle with ...
Malaspina, Francesco +5 more
core +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells
FEBS Open Bio, EarlyView.BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley +1 more source
Methodological approaches to differential detection of EBV1/EBV2 and HHV6A/HHV6B in saliva
Инфекция и иммунитет, 2019 Epstein-Barr virus (EBV) and human herpesviruses 6A and 6B (HHV6A and HHV6B) are ubiquitous, infecting all social groups. In Russia, information on the genetic heterogeneity of EBV, even at the level of the main types (EBV1 and EBV2), as well as HHV6A ...
Mariia Igorevna Popkova +7 more
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