Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Reliability Assessment Methodology for Massive Manufacturing Using Multi-Function Equipment
Complexity, 2018 Experience reveals that reliability varies depending on the characteristics of operation. The manufacturing process based on multifunction equipment gives a usual case of variation in operating conditions.
M. López-Campos +3 more
doaj +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
ADVANCES IN RELIABILITY OPTIMIZATION OF MULTI-STATE SYSTEM
Jixie qiangdu, 2016 Multi-state system reliability is very important for complex systems which have a range of performance levels from perfect functioning to complete failure.
LI ChunYang, CHEN ShouHua, LI LingWei
doaj
Universal Generator for Complementary Pairs of Sequences Based on Boolean Functions
CoRR, 2013 We present a general algorithm for generating arbitrary standard complementary pairs of sequences (including binary, polyphase, M-PSK and QAM) of length 2^N using Boolean functions. The algorithm follows our earlier paraunitary algorithm, but does not require matrix multiplications.
Srdjan Z. Budisin, Predrag Spasojevic
openaire +3 more sources
Pointwise universal consistency of nonparametric density estimators. [PDF]
This paper presents sufficient conditions for pointwise universal consistency of nonparametric delta estimators and shows the application of these conditions for some classes of nonparametric estimators.Delta estimators; Pointwise approximation ...
Vidal-Sanz, Jose M.
core
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
FEBS Open Bio, EarlyView.Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy
FEBS Open Bio, EarlyView.This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan +11 more
wiley +1 more source
Pointwise universal consistency of nonparametric linear estimators [PDF]
, 2004 This paper presents sufficient conditions for pointwise universal consistency of nonparametric delta estimators.
Vidal-Sanz, Jose M.
core +1 more source