Results 11 to 20 of about 2,845,268 (289)

Trifluoroselenomethionine: A New Unnatural Amino Acid [PDF]

ChemBioChem, 2016
AbstractTrifluoroselenomethionine (TFSeM), a new unnatural amino acid, was synthesized in seven steps from N‐(tert‐butoxycarbonyl)‐l‐aspartic acid tert‐butyl ester. TFSeM shows enhanced methioninase‐induced cytotoxicity, relative to selenomethionine (SeM), toward HCT‐116 cells derived from human colon cancer.
Eric Block, Squire J. Booker, Sonia Flores‐Penalba, Graham N. George, Sivaji Gundala, Bradley J. Landgraf, Jun Liu, Stephene N. Lodge, M. Jake Pushie, Sharon Rozovsky, Abith Vattekkatte, Rama Yaghi, Huawei Zeng   +12 more
openaire   +3 more sources

Development and Testing of Force Field Parameters for Phenylalanine and Tyrosine Derivatives

Frontiers in Molecular Biosciences, 2020
Theoretical analyses are valuable for the exploration of the effects of unnatural amino acids on enzyme functions; however, many necessary parameters for unnatural amino acids remain lacking.
Xiaowen Wang, Xiaowen Wang, Wenjin Li
doaj   +1 more source

Enzymatic Screening of β-Amyloid Precursor Protein-Based Substrates

Compounds, 2023
We performed an enzymatic screening of synthetic peptides based on β-amyloid precursor protein substrates. The template peptide sequence was a decapeptide derived from our previous screening study, which determined several effective unnatural amino acids.
Reo Yamada, Masaki Midorikawa, Ayu Asai, Norimasa Takasu, Ren Fujii, Taeko Kakizawa   +5 more
doaj   +1 more source

Genetic Code Engineering by Natural and Unnatural Base Pair Systems for the Site-Specific Incorporation of Non-Standard Amino Acids Into Proteins

Frontiers in Molecular Biosciences, 2022
Amino acid sequences of proteins are encoded in nucleic acids composed of four letters, A, G, C, and T(U). However, this four-letter alphabet coding system limits further functionalities of proteins by the twenty letters of amino acids.
Michiko Kimoto, Ichiro Hirao
doaj   +1 more source

A mutation in amino acid permease AAP6 reduces the amino acid content of the Arabidopsis sieve elements but leaves aphid herbivores unaffected [PDF]

, 2010
The aim of this study was to investigate the role of the amino acid permease gene AAP6 in regulating phloem amino acid composition and then to determine the effects of this altered diet on aphid performance.
Newbury, H. J., Hunt, E. (Emma), Gattolin, Stefano, Pritchard, J. (Jeremy), Tseng, Hua-Ming, Bale, J. S. (Jeffrey S.), Barrett, D. A. (David A.)   +6 more
core   +2 more sources

Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids

Nature Communications, 2023
The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant ...
Yunjun Pan, Guobang Li, Ruxin Liu, Jiawei Guo, Yunjie Liu, Mingyu Liu, Xingwang Zhang, Luping Chi, Kangwei Xu, Ruibo Wu, Yuzhong Zhang, Yuezhong Li, Xiang Gao, Shengying Li   +13 more
doaj   +1 more source

Genetically incorporated crosslinkers reveal NleE attenuates host autophagy dependent on PSMD10

eLife, 2021
Autophagy acts as a pivotal innate immune response against infection. Some virulence effectors subvert the host autophagic machinery to escape the surveillance of autophagy.
Jingxiang Li, Shupan Guo, Fangni Chai, Qi Sun, Pan Li, Li Gao, Lunzhi Dai, Xiaoxiao Ouyang, Zhihui Zhou, Li Zhou, Wei Cheng, Shiqian Qi, Kefeng Lu, Haiyan Ren   +13 more
doaj   +1 more source

Substrates incorporating unnatural amino acids are separately detectable for chymotrypsin and subtilisin with similar specificity

Results in Chemistry, 2023
Protease specificity depends on the three-dimensional structure and sequence of the protease, and each protease plays a different role. Chymotrypsin and subtilisin have similar specificity for recognizing aromatic and hydrophobic amino acid residues ...
Yuuki Yamawaki, Tamaki Kato
doaj   +1 more source

Genetic incorporation of unnatural amino acids into proteins in Mycobacterium tuberculosis. [PDF]

PLoS ONE, 2010
New tools are needed to study the intracellular pathogen Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), to facilitate new drug discovery and vaccine development.
Feng Wang, Scott Robbins, Jiantao Guo, Weijun Shen, Peter G Schultz   +4 more
doaj   +1 more source

In Vivo Incorporation of Multiple Unnatural Amino Acids [PDF]

, 2009
Unnatural amino acid (UAA) incorporation is an invaluable technique that is seeing increased use. THG73 is an amber suppressor tRNA used to incorporate > 100 residues at the UAG, amber stop codon, in Xenopus oocytes.
Rodriguez, Erik Ali
core   +1 more source