Results 21 to 30 of about 2,845,268 (289)

Unnatural amino acid incorporation in E. coli: current and future applications in the design of therapeutic proteins.

Frontiers in Chemistry, 2014
Unnatural amino acid (UAA) incorporation by amber codon suppression offers scientists a powerful tool to modify the properties of proteins at will. UAA incorporation has been used for a plethora of fundamental research applications and, more recently ...
Huib eOvaa, kim ewals
doaj   +1 more source

Structure-Function Studies of Nicotinic Acetylcholine Receptors Using Unnatural Amino Acids [PDF]

, 2012
This dissertation primarily describes structure-function studies of the nicotinic acetylcholine receptors (nAChRs). These studies use a combination of unnatural amino acid mutagenesis and electrophysiology to determine the specific molecular interactions
Puskar, Nyssa Leigh
core   +1 more source

Bicyclic Pyrrolidine-Isoxazoline γ Amino Acid: A Constrained Scaffold for Stabilizing α-Turn Conformation in Isolated Peptides

Frontiers in Chemistry, 2019
Unnatural amino acids have tremendously expanded the folding possibilities of peptides and peptide mimics. While α,α-disubstituted and β-amino acids are widely studied, γ-derivatives have been less exploited.
Francesco Oliva, Raffaella Bucci, Lucia Tamborini, Stefano Pieraccini, Andrea Pinto, Sara Pellegrino   +5 more
doaj   +1 more source

Evaluation and optimisation of unnatural amino acid incorporation and bioorthogonal bioconjugation for site-specific fluorescent labelling of proteins expressed in mammalian cells

Biochemistry and Biophysics Reports, 2019
Many biophysical techniques that are available to study the structure, function and dynamics of cellular constituents require modification of the target molecules.
Leonhard Jakob, Alexander Gust, Dina Grohmann   +2 more
doaj   +1 more source

Site-Specific Antibody Conjugation with Payloads beyond Cytotoxins

Molecules, 2023
As antibody–drug conjugates have become a very important modality for cancer therapy, many site-specific conjugation approaches have been developed for generating homogenous molecules.
Qun Zhou
doaj   +1 more source

Activation of Prp28 ATPase by phosphorylated Npl3 at a critical step of spliceosome remodeling

Nature Communications, 2021
Yeast helicase Prp28 promotes the first step of spliceosome remodeling. By placing a photoactivatable unnatural amino acid in Prp28, the authors capture Prp28 in action revealing its dynamic interactions and cofactor Npl3.
Fu-Lung Yeh, Shang-Lin Chang, Golam Rizvee Ahmed, Hsin-I Liu, Luh Tung, Chung-Shu Yeh, Leah Stands Lanier, Corina Maeder, Che-Min Lin, Shu-Chun Tsai, Wan-Yi Hsiao, Wei-Hau Chang, Tien-Hsien Chang   +12 more
doaj   +1 more source

Antimicrobial Activity of Amino Acid Analogues and Their Derivatives

Proceedings, 2017
Libraries of compounds containing unnatural amino acids or amino acid analogues were prepared from inexpensive substrates using sustainable processes [1–3]. [...]
Dácil Hernández, Carlos Saavedra, Carmen Carro, Alicia Boto, José M. Pérez de Lastra   +4 more
doaj   +1 more source

Enzymatic aminoacylation of tRNA with unnatural amino acids [PDF]

Proceedings of the National Academy of Sciences, 2006
The biochemical flexibility of the cellular translation apparatus offers, in principle, a simple route to the synthesis of drug-like modified peptides and novel biopolymers. However, only ≈75 unnatural building blocks are known to be fully compatible with enzymatic tRNA acylation and subsequent ribosomal synthesis of modified ...
Matthew C T, Hartman, Kristopher, Josephson, Jack W, Szostak   +2 more
openaire   +2 more sources

Synthesis of Both Enantiomers of Chiral Phenylalanine Derivatives Catalyzed by Cinchona Alkaloid Quaternary Ammonium Salts as Asymmetric Phase Transfer Catalysts

Molecules, 2018
A practical synthesis of both enantiomers of unnatural phenylalanine derivatives by using two pseudoenantiomeric phase transfer catalysts is described.
Lei Jin, Shuai Zhao, Xin Chen
doaj   +1 more source

A New Unnatural Amino Acid Derived from the Modification of 4′-(p-tolyl)-2,2′:6′,2″-terpyridine and Its Mixed-Ligand Complexes with Ruthenium: Synthesis, Characterization, and Photophysical Properties

Chemistry, 2023
The modification of the methyl group of 4′-(p-tolyl)-2,2′:6′,2″-terpyridine produced the novel unnatural amino acid 3-(4-([2,2′:6′,2″-terpyridin]-4′-yl)phenyl)-2-aminopropanoic acid (phet).
Konstantinos Ypsilantis, Antonia Garypidou, Andreas Gikas, Alexandros Kiapekos, John C. Plakatouras, Achilleas Garoufis   +5 more
doaj   +1 more source