Efficient Incorporation of Unnatural Amino Acids into Proteins with a Robust Cell-Free System
Methods and Protocols, 2019 Unnatural proteins are crucial biomacromolecules and have been widely applied in fundamental science, novel biopolymer materials, enzymes, and therapeutics.
Wei Gao, Ning Bu, Yuan Lu
doaj +1 more source
Visualizing HCV Core Protein via Fluorescent Unnatural Amino Acid Incorporation
Proceedings, 2020 Introduction: Unnatural amino acids (UAAs) share the same basic structure as proteinogenic amino acids. However, UAAs permit additional functions and applications to proteins due to their different side chains.
Leah Jane Fitzgerald Curnew +8 more
doaj +1 more source
Recent advances in the synthesis and application of fluorescent α-amino acids [PDF]
, 2016 Fluorescence spectroscopy has become a powerful technique for probing a range of complex biological processes including enzyme mechanisms and protein-protein interactions. While the application of this technique uses a number of strategies, many of these
Harkiss, Alexander, Sutherland, Andrew
core +1 more source
Development of natural and unnatural amino acid delivery systems against hookworm infection
Precision Nanomedicine, 2020 Peptide-based vaccines consist of short antigen fragments derived from a specific pathogen. Alone, these peptide fragments are poorly or non-immunogenic; however, when incorporated into a proper delivery system, they can trigger strong immune responses.
Stacey Bartlett +5 more
doaj +1 more source
Synthesis of an Unnatural Fluorescent Amino Acid [PDF]
, 2019 See the ...
Niemeyer, Cassandra
core +4 more sources
ACS Synthetic Biology, 2018 Tight regulatory system for gene expression, which is ideally controlled by unnatural and bio-orthogonal substances, is a keystone for successful construction of synthetic gene circuits.
Y. Kato
semanticscholar +1 more source
Chemoenzymatic synthesis of polypeptides containing the unnatural amino acid 2-aminoisobutyric acid.
Chemical Communications, 2017 Polypeptides containing 2-aminoisobutyric acid (Aib) units as an unnatural amino acid residue were synthesized by papain-catalyzed chemoenzymatic polymerization of a tripeptide ethyl ester l-Ala-Aib-l-Ala-OEt in an aqueous medium.
K. Tsuchiya, K. Numata
semanticscholar +1 more source
Synthesis of Isothiocyanates Using DMT/NMM/TsO− as a New Desulfurization Reagent
Molecules, 2021 Thirty-three alkyl and aryl isothiocyanates, as well as isothiocyanate derivatives from esters of coded amino acids and from esters of unnatural amino acids (6-aminocaproic, 4-(aminomethyl)benzoic, and tranexamic acids), were synthesized with ...
Łukasz Janczewski +2 more
doaj +1 more source
Protein-based materials, toward a new level of structural control [PDF]
, 2001 Through billions of years of evolution nature has created and refined structural proteins for a wide variety of specific purposes. Amino acid sequences and their associated folding patterns combine to create elastic, rigid or tough materials.
Tirrell, David A., van Hest, Jan C. M.
core +1 more source
Electrostatic Contributions of Aromatic Residues in the Local Anesthetic Receptor of Voltage-Gated Sodium Channels [PDF]
, 2008 Antiarrhythmics, anticonvulsants, and local anesthetics target voltage-gated sodium channels, decreasing excitability of nerve and muscle cells. Channel inhibition by members of this family of cationic, hydrophobic drugs relies on the presence of highly ...
Ahern, Christopher A. +3 more
core +2 more sources