Results 141 to 150 of about 3,915 (172)

Uridine diphosphate galactose 4-epimerase in human and other mammalian hemolysates

Biochimica et Biophysica Acta (BBA) - Enzymology, 1973
Abstract 1. 1. In contrast to the requirement for exogenous NAD+ in the assay of UDP-galactose 4-epimerase (UDPgalactose epimerase, EC 5.1.3.2), in hemolysates from adults, substantial epimerase activity can be demonstrated in hemolysates from newborn infants without addition of NAD+.
William R. Bergren, Won G. Ng, George N. Donnell   +2 more
openaire   +1 more source

Inhibition of uridine diphosphate glucose dehydrogenase by metabolic intermediates of galactose

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1964
Abstract UDPG dehydrogenase (EC 1.1.1.22) from calf liver is competitively inhibited by UDPGal in vitro and “uncompetitive” inhibition of the enzyme is shown by UDP. The K i for UDPGal is 1.33 · 10 −4 M, the K m for UDPG is 0.9–1.2 · 10 −5 M at pH 8.3.
G. Salitis, I.T. Oliver
openaire   +1 more source

HPLC analysis of uridine diphosphate sugars: decreased concentrations of uridine diphosphate galactose in erythrocytes and cultured skin fibroblasts from classical galactosemia patients

Clinica Chimica Acta, 1995
Abstract A high performance liquid chromatography (HPLC) method has been developed for the measurement of uridine diphosphate galactose (UDPGal) and uridine diphosphate glucose (UDPGlc) in erythrocytes and cultured skin fibroblasts of normal controls and galactosemia patients.
Y K, Xu, F R, Kaufman, G N, Donnell, T, Giudici, O, Alfi, W G, Ng   +5 more
openaire   +2 more sources

Efficient One-Pot Synthesis of Uridine Diphosphate Galactose Employing a Trienzyme System

Journal of Agricultural and Food Chemistry
The limited availability of high-cost nucleotide sugars is a significant constraint on the application of their downstream products (glycosides and prebiotics) in the food or pharmaceutical industry. To better solve the problem, this study presented a one-pot approach for the biosynthesis of UDP-Gal using a thermophilic multienzyme system consisting of
Yajing Li, Qi Chen, Siyao Liu, Lin Deng, Shichao Li, Renjun Gao   +5 more
openaire   +2 more sources

A simple assay for uridine diphosphate galactose 4-epimerase activity.

Annales de genetique, 1980
A simple fluorescent test for the activity of blood uridine diphosphate galactose 4-epimerase which converts uridine diphosphate galactose to uridine diphosphate glucose is described. The enzyme activity is visually estimated by to reduction of NAD+ (non fluorescent) to NADH (fluorescent) in a coupled reaction with uridine diphosphate glucose ...
G, Vaca, J, Sanchez-Corona, N, Olivares, C, Medina, B, Ibarra, J M, Cantu   +5 more
openaire   +1 more source

Competitive inhibition and substrate activity of uridine diphosphate 6-deoxygalactose for Escherichia coli uridine diphosphate galactose 4-epimerase.

The Biochemical journal, 1973
UDP-6-deoxygalactose inhibits the UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli in a competitive manner with respect to the substrate UDP-galactose, giving K(i) 1.3x10(-3)m. As a substrate for the enzyme, it is transformed into UDP-6-deoxyglucose, although the reaction stops before equilibrium is attained.
M, Spencer, P, Blackburn, W, Ferdinand, G M, Blackburn   +3 more
openaire   +1 more source

Interaction between uridine diphosphate galactose and uridine diphosphate galactose 4-epimerase from Escherichia coli

Biochemistry, 1972
Y, Seyama, H M, Kalckar
openaire   +2 more sources

[Cold storage of rabbit platelet suspension by adding uridine diphosphate galactose].

Zhongguo shi yan xue ye xue za zhi, 2007
This study was aimed to investigate the method to cold-store platelets with uridine diphosphate galactose (UDP-Gal). Rabbit heart blood was prepared for concentrated platelet suspension to which UDP-Gal was added, and then stored for ten days in 4 degrees C refrigerator.
Xiao-Ping, Pei, Bao-An, Chen, Cheng-Yin, Huang, Cui-Ping, Li, Guang-Yao, Shi, Jian-Yu, Xiao, Chong, Gao, Jia-Hua, Ding, Fei, Fei, Jun, Wang, Yun-Yu, Sun, Jian, Cheng, Gang, Zhao   +12 more
openaire   +1 more source

Concerted reduction of yeast uridine diphosphate galactose 4-epimerase

Biochemistry, 1971
A U, Bertland, Y, Seyama, H M, Kalckar
openaire   +2 more sources

Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering

Nature Communications, 2021
William Birmingham, Asbjørn Toftgaard Pedersen, Mafalda Dias Gomes   +2 more
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