Results 1 to 10 of about 107,812 (233)
RNA structural probing of guanine and uracil nucleotides in yeast. [PDF]
PLoS ONE, 2023 RNA structure can be essential for its cellular function. Therefore, methods to investigate the structure of RNA in vivo are of great importance for understanding the role of cellular RNAs.
Kevin Xiao +2 more
doaj +4 more sources
Involvement of pyrimidinoceptors in the regulation of cell functions by uridine and by uracil nucleotides [PDF]
Trends in Pharmacological Sciences, 1989 Uridine and uracil nucleotides are involved in the regulation of various cell functions. Here, Roland Seifert and Günter Schultz review the evidence that, rather than by binding to purinoceptors, pyrimidine nucleotides exert their effects by binding to distinct pyrimidinoceptors, which are coupled to pertussis toxin-sensitive G proteins in human ...
Gunter Schultz
exaly +7 more sources
Genetic analysis of pyrimidine biosynthetic enzymes in Plasmodium falciparum. [PDF]
PLoS PathogensThe malaria parasite Plasmodium falciparum depends entirely on de novo pyrimidine synthesis, as it is unable to salvage these essential nucleotides. This reliance makes the pyrimidine biosynthesis pathway a compelling target for antimalarial drugs, with ...
Krithika Rajaram +6 more
doaj +2 more sources
Is GPR17 a P2Y/leukotriene receptor? examination of uracil nucleotides, nucleotide sugars, and cysteinyl leukotrienes as agonists of GPR17. [PDF]
J Pharmacol Exp Ther, 2013 The orphan receptor GPR17 has been reported to be activated by UDP, UDP-sugars, and cysteinyl leukotrienes, and coupled to intracellular Ca2+ mobilization and inhibition of cAMP accumulation, but other studies have reported either a different agonist profile or lack of agonist activity altogether.
Qi AD, Harden TK, Nicholas RA.
europepmc +4 more sources
The nucleobase analog 4-thiouracil hijacks the pyrimidine salvage pathway to inhibit Staphylococcus aureus growth [PDF]
Microbiology SpectrumStaphylococcus aureus is a leading cause of bacterial-induced mortality due to infections that are increasingly resistant to antibiotics, highlighting the need for new therapeutic strategies to treat these drug-resistant infections.
Matthew J. Munneke +2 more
doaj +2 more sources
UdgX-Mediated Uracil Sequencing at Single-Nucleotide Resolution [PDF]
Journal of the American Chemical Society, 2022 As an aberrant base in DNA, uracil is generated by either deoxyuridine (dU) misincorporation or cytosine deamination, and involved in multiple physiological and pathological processes. Genome-wide profiles of uracil are important for study of these processes.
Liudan Jiang +19 more
openaire +4 more sources
BioTechniques, 2022 This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase.
Naoki Harikai +4 more
doaj +1 more source
On the prebiotic selection of nucleotide anomers: A computational study
Heliyon, 2022 Present-day known predominance of the β- over the α-anomers in nucleosides and nucleotides emerges from a thermodynamic analysis of their assembly from their components, i.e. bases, sugars, and a phosphate group.
Lázaro A.M. Castanedo, Chérif F. Matta
doaj +1 more source
Biochemical characterization of uracil phosphoribosyltransferase from Mycobacterium tuberculosis. [PDF]
PLoS ONE, 2013 Uracil phosphoribosyltransferase (UPRT) catalyzes the conversion of uracil and 5-phosphoribosyl-α-1-pyrophosphate (PRPP) to uridine 5'-monophosphate (UMP) and pyrophosphate (PP(i)). UPRT plays an important role in the pyrimidine salvage pathway since UMP
Anne Drumond Villela +8 more
doaj +1 more source
RNA Biology, 2023 Here, we provide an in-depth analysis of the usefulness of single-sample metabolite/RNA extraction for multi-‘omics readout. Using pulverized frozen livers of mice injected with lymphocytic choriomeningitis virus (LCMV) or vehicle (Veh), we isolated RNA ...
Zachary B Madaj +13 more
doaj +1 more source