Results 11 to 20 of about 669,704 (210)

UdgX-Mediated Uracil Sequencing at Single-Nucleotide Resolution [PDF]

Journal of the American Chemical Society, 2022
As an aberrant base in DNA, uracil is generated by either deoxyuridine (dU) misincorporation or cytosine deamination, and involved in multiple physiological and pathological processes. Genome-wide profiles of uracil are important for study of these processes.
Liudan Jiang, Jiayong Yin, Maoxiang Qian, Shaoqin Rong, Shengqi Zhang, Kejing Chen, Chengchen Zhao, Yuanqing Tan, Jiayin Guo, Hao Chen, Siyun Gao, Tingting Liu, Yi Liu, Bin Shen, Jian Yang, Yong Zhang, Fei-Long Meng, Jinchuan Hu, Honghui Ma, Yi-Han Chen   +19 more
openaire   +4 more sources

Methanocarba Modification of Uracil and Adenine Nucleotides:  High Potency of Northern Ring Conformation at P2Y1, P2Y2, P2Y4, and P2Y11but Not P2Y6Receptors

Journal of Medicinal Chemistry, 2002
Kenneth A Jacobson, Malin Malmsjö, David Erlinge   +2 more
exaly   +2 more sources

Real-time PCR method for detection of short DNA using a deoxyuridine probe and application for detection of fomivirsen

BioTechniques, 2022
This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase.
Naoki Harikai, Yuko Tanaka, Satoshi Miyashita, Kazumasa Zaima, Kazufusa Shinomiya   +4 more
doaj   +1 more source

On the prebiotic selection of nucleotide anomers: A computational study

Heliyon, 2022
Present-day known predominance of the β- over the α-anomers in nucleosides and nucleotides emerges from a thermodynamic analysis of their assembly from their components, i.e. bases, sugars, and a phosphate group.
Lázaro A.M. Castanedo, Chérif F. Matta
doaj   +1 more source

Biochemical characterization of uracil phosphoribosyltransferase from Mycobacterium tuberculosis. [PDF]

PLoS ONE, 2013
Uracil phosphoribosyltransferase (UPRT) catalyzes the conversion of uracil and 5-phosphoribosyl-α-1-pyrophosphate (PRPP) to uridine 5'-monophosphate (UMP) and pyrophosphate (PP(i)). UPRT plays an important role in the pyrimidine salvage pathway since UMP
Anne Drumond Villela, Rodrigo Gay Ducati, Leonardo Astolfi Rosado, Carlos Junior Bloch, Maura Vianna Prates, Danieli Cristina Gonçalves, Carlos Henrique Inacio Ramos, Luiz Augusto Basso, Diogenes Santiago Santos   +8 more
doaj   +1 more source

Genetic predisposition to porto‐sinusoidal vascular disorder: A functional genomic‐based, multigenerational family study

Hepatology, EarlyView., 2022
A deleterious variant of FCHSD1 results in mTOR pathway overactivation and may cause porto‐sinusoidal vascular disorder (PSVD). The pedigree of the family demonstrated an autosomal dominant disease with variable expressivity. Whole‐genome sequencing and Sanger sequencing both validated the existence of the FCHSD1 variant and the heterozygosity of c ...
Jingxuan Shan, André Megarbane, Aziz Chouchane, Deepak Karthik, Ramzi Temanni, Atilio Reyes Romero, Huiying Hua, Chun Pan, Xixi Chen, Murugan Subramanian, Chadi Saad, Hamdi Mbarek, Cybel Mehawej, Eliane Chouery, Sirin W. Abuaqel, Alexander Dömling, Sami Remadi, Cesar Yaghi, Pu Li, Lotfi Chouchane   +19 more
wiley   +1 more source

Prior metabolite extraction fully preserves RNAseq quality and enables integrative multi-‘omics analysis of the liver metabolic response to viral infection

RNA Biology, 2023
Here, we provide an in-depth analysis of the usefulness of single-sample metabolite/RNA extraction for multi-‘omics readout. Using pulverized frozen livers of mice injected with lymphocytic choriomeningitis virus (LCMV) or vehicle (Veh), we isolated RNA ...
Zachary B Madaj, Michael S. Dahabieh, Vijayvardhan Kamalumpundi, Brejnev Muhire, J. Pettinga, Rebecca A. Siwicki, Abigail E. Ellis, Christine Isaguirre, Martha L. Escobar Galvis, Lisa DeCamp, Russell G. Jones, Scott A. Givan, Marie Adams, Ryan D. Sheldon   +13 more
doaj   +1 more source

Silencing NTPDase3 activity rehabilitates the osteogenic commitment of post-menopausal stem cell bone progenitors

Stem Cell Research & Therapy, 2023
Background Endogenously released adenine and uracil nucleotides favour the osteogenic commitment of bone marrow-derived mesenchymal stromal cells (BM-MSCs) through the activation of ATP-sensitive P2X7 and UDP-sensitive P2Y6 receptors.
José Bernardo Noronha-Matos, Rui Pinto-Cardoso, Catarina Bessa-Andrês, Maria Teresa Magalhães-Cardoso, Fátima Ferreirinha, Maria Adelina Costa, José Marinhas, Rolando Freitas, Rui Lemos, Adélio Vilaça, António Oliveira, Julie Pelletier, Jean Sévigny, Paulo Correia-de-Sá   +13 more
doaj   +1 more source

Hydrophilic interaction liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry for determination of nuclear and cytoplasmatic contents of nucleotides, nucleosides and their nucleobases in food yeasts

Talanta Open, 2021
Hydrophilic interaction liquid chromatography using a zwitterionic phase, coupled with high-resolution mass spectrometry based on quadrupole and time-of-flight mass analyser (HILIC-Q-TOF-MS) is applied for the determination of four nucleobases (adenine ...
Marta Pastor-Belda, Inmaculada Fernández-Caballero, Natalia Campillo, Natalia Arroyo-Manzanares, Manuel Hernández-Córdoba, Pilar Viñas   +5 more
doaj   +1 more source

Statistical analysis of readthrough levels for nonsense mutations in mammalian cells reveals a major determinant of response to gentamicin. [PDF]

PLoS Genetics, 2012
The efficiency of translation termination depends on the nature of the stop codon and the surrounding nucleotides. Some molecules, such as aminoglycoside antibiotics (gentamicin), decrease termination efficiency and are currently being evaluated for ...
Célia Floquet, Isabelle Hatin, Jean-Pierre Rousset, Laure Bidou   +3 more
doaj   +1 more source