Results 31 to 40 of about 669,704 (210)

Nonsynonymous Mutations in Linker-2 of the Pdr5 Multidrug Transporter Identify a New RNA Stability Element

G3: Genes, Genomes, Genetics, 2020
Analysis of synonymous mutations established that although the primary amino acid sequence remains unchanged, alterations in transcription and translation can result in significant phenotypic consequences. We report the novel observation that a series of
Hadiar Rahman, Andrew Rudrow, Joshua Carneglia, Sister Stephen Patrick Joly, Dante Nicotera, Michael Naldrett, John Choy, Suresh V. Ambudkar, John Golin   +8 more
doaj   +1 more source

Crystal structure and functional implications of cyclic di-pyrimidine-synthesizing cGAS/DncV-like nucleotidyltransferases

Nature Communications, 2023
Purine-containing nucleotide second messengers regulate diverse cellular activities. Cyclic di-pyrimidines mediate anti-phage functions in bacteria; however, the synthesis mechanism remains elusive.
Chia-Shin Yang, Tzu-Ping Ko, Chao-Jung Chen, Mei-Hui Hou, Yu-Chuan Wang, Yeh Chen   +5 more
doaj   +1 more source

Expression of microRNAs in Horse Plasma and Their Characteristic Nucleotide Composition. [PDF]

PLoS ONE, 2016
MicroRNAs (miRNAs) in blood plasma are stable under high levels of ribonuclease activity and could function in tissue-to-tissue communication, suggesting that they may have distinctive structural characteristics compared with non-circulating miRNAs.
Seungwoo Lee, Seungwoo Hwang, Hee Jeong Yu, Dayoung Oh, Yu Jung Choi, Myung-Chul Kim, Yongbaek Kim, Doug-Young Ryu   +7 more
doaj   +1 more source

High expression of uracil DNA glycosylase determines C to T substitution in human pluripotent stem cells

Molecular Therapy: Nucleic Acids, 2022
Precise genome editing of human pluripotent stem cells (hPSCs) is crucial not only for basic science but also for biomedical applications such as ex vivo stem cell therapy and genetic disease modeling.
Ju-Chan Park, Hyeon-Ki Jang, Jumee Kim, Jun Hee Han, Youngri Jung, Keuntae Kim, Sangsu Bae, Hyuk-Jin Cha   +7 more
doaj   +1 more source

The epitranscriptome in stem cell biology and neural development

Neurobiology of Disease, 2020
The blossoming field of epitranscriptomics has recently garnered attention across many fields by findings that chemical modifications on RNA have immense biological consequences.
Caroline Vissers, Aniketa Sinha, Guo-li Ming, Hongjun Song   +3 more
doaj   +1 more source

Northern Blot of tRNA in Yeast

Bio-Protocol, 2013
tRNAs are small RNAs around 70-90 nt. tRNAs are different from many other small RNAs in that they are very abundant, which makes it difficult to study their transcriptional regulation by traditional northern blot.
Yuehua Wei
doaj   +1 more source

Effects of vaccinia virus uracil DNA glycosylase catalytic site and deoxyuridine triphosphatase deletion mutations individually and together on replication in active and quiescent cells and pathogenesis in mice

Virology Journal, 2008
Background Low levels of uracil in DNA result from misincorporation of dUMP or cytosine deamination. Vaccinia virus (VACV), the prototype poxvirus, encodes two enzymes that can potentially reduce the amount of uracil in DNA.
Moss Bernard, De Silva Frank S
doaj   +1 more source

Molecular characterization of the virulent infectious hematopoietic necrosis virus (IHNV) strain 220-90

Virology Journal, 2010
Background Infectious hematopoietic necrosis virus (IHNV) is the type species of the genus Novirhabdovirus, within the family Rhabdoviridae, infecting several species of wild and hatchery reared salmonids.
LaPatra Scott E, Ammayappan Arun, Vakharia Vikram N   +2 more
doaj   +1 more source

Transcriptional reprogramming of nucleotide metabolism in response to altered pyrimidine availability in Arabidopsis seedlings

Frontiers in Plant Science, 2023
In Arabidopsis seedlings, inhibition of aspartate transcarbamoylase (ATC) and de novo pyrimidine synthesis resulted in pyrimidine starvation and developmental arrest a few days after germination.
Robert D. Slocum, Carolina Mejia Peña, Zhongchi Liu   +2 more
doaj   +1 more source

Metabolome analysis of genus Forsythia related constituents in Forsythia suspensa leaves and fruits using UPLC-ESI-QQQ-MS/MS technique.

PLoS ONE, 2022
Forsythia suspensa is a traditional Chinese herb. Its numerous metabolites have important roles, as they possessed a wide range of biological activities. This study explored the accumulations of F.
Lingdi Liu, Yu Sun, Chunxiu Wen, Tao Jiang, Wei Tian, Xiaoliang Xie, Xusheng Cui, Ruike Lu, Jiaxing Feng, Aihong Jin, Saiqun Wen, Wei Wei   +11 more
doaj   +1 more source