Results 31 to 40 of about 107,812 (233)
Cytosine-to-Uracil Deamination by SssI DNA Methyltransferase [PDF]
, 2013 The prokaryotic DNA(cytosine-5)methyltransferase M.SssI shares the specificity of eukaryotic DNA methyltransferases (CG) and is an important model and experimental tool in the study of eukaryotic DNA methylation.
Stier, Ildikó +5 more
core +2 more sources
Expression of microRNAs in Horse Plasma and Their Characteristic Nucleotide Composition. [PDF]
PLoS ONE, 2016 MicroRNAs (miRNAs) in blood plasma are stable under high levels of ribonuclease activity and could function in tissue-to-tissue communication, suggesting that they may have distinctive structural characteristics compared with non-circulating miRNAs.
Seungwoo Lee +7 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2022 Precise genome editing of human pluripotent stem cells (hPSCs) is crucial not only for basic science but also for biomedical applications such as ex vivo stem cell therapy and genetic disease modeling.
Ju-Chan Park +7 more
doaj +1 more source
The epitranscriptome in stem cell biology and neural development
Neurobiology of Disease, 2020 The blossoming field of epitranscriptomics has recently garnered attention across many fields by findings that chemical modifications on RNA have immense biological consequences.
Caroline Vissers +3 more
doaj +1 more source
Tools and drugs for uracil nucleotide-activated P2Y receptors
Pharmacology & Therapeutics, 2018 P2Y receptors (P2YRs) are a family of G protein-coupled receptors activated by extracellular nucleotides. Physiological P2YR agonists include purine and pyrimidine nucleoside di- and triphosphates, such as ATP, ADP, UTP, UDP, nucleotide sugars, and dinucleotides.
Rafehi, Muhammad, Müller, Christa E
openaire +4 more sources
Characterization of Escherichia coli double-strand uracil-DNA glycosylase and analysis of uracil-initiated base excision DNA repair [PDF]
Escherichia coli double-strand uracil-DNA glycosylase (Dug) was purified to apparent homogeneity from bacteria that were defective in uracil-DNA glycosylase (Ung). After cloning the dug gene, recombinant Dug was overexpressed, purified, and characterized
Sung, Jung-Suk
core +6 more sources
Northern Blot of tRNA in Yeast
Bio-Protocol, 2013 tRNAs are small RNAs around 70-90 nt. tRNAs are different from many other small RNAs in that they are very abundant, which makes it difficult to study their transcriptional regulation by traditional northern blot.
Yuehua Wei
doaj +1 more source
Turning On Uracil-DNA Glycosylase Using a Pyrene Nucleotide Switch [PDF]
Journal of Biological Chemistry, 2001 Base flipping is a highly conserved process by which enzymes swivel an entire nucleotide from the DNA base stack into their active site pockets. Uracil DNA glycosylase (UDG) is a paradigm enzyme that uses a base flipping mechanism to catalyze the hydrolysis of the N-glycosidic bond of 2'-deoxyuridine (2'-dUrd) in DNA as the first step in uracil base ...
Y L, Jiang, K, Kwon, J T, Stivers
openaire +2 more sources
Simultaneous quantification of 12 different nucleotides and nucleosides released from renal epithelium and in human urine samples using ion-pair reversed-phase HPLC [PDF]
, 2012 Nucleotides and nucleosides are not only involved in cellular metabolism but also act extracellularly via P1 and P2 receptors, to elicit a wide variety of physiological and pathophysiological responses through paracrine and autocrine signalling pathways.
Claire M. Peppiatt-Wildman +18 more
core +1 more source
The Orphan Receptor GPR17 Is Unresponsive to Uracil Nucleotides and Cysteinyl Leukotrienes [PDF]
Molecular Pharmacology, 2017 Pairing orphan G protein–coupled receptors (GPCRs) with their cognate endogenous ligands is expected to have a major impact on our understanding of GPCR biology. It follows that the reproducibility of orphan receptor ligand pairs should be of fundamental importance to guide meaningful investigations into the pharmacology and function of individual ...
Katharina, Simon +12 more
openaire +2 more sources