Results 111 to 120 of about 1,886,665 (203)

Meta-analysis of functional genomics studies reveals conserved cellular pathways required by viruses of pandemic concern. [PDF]

Access Microbiol
Brice AM, Caputo AT, Stewart CR.
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Discovery of a novel emaravirus and an alphacytorhabdovirus infecting Spiraea in the USA. [PDF]

Arch Virol
Serrano Salgado J, Vera M, Keener R, Gray J, Arenz B, Mollov D, Kilmov PB, Alvarez-Quinto R.   +7 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Risk Prediction Models for Recurrence After Curative Treatment of Early‐Stage or Locally Advanced Lung Cancer: A Systematic Review

Aging and Cancer, EarlyView.
This systematic review synthesizes prognostic models for survival and recurrence in resected non‐small cell lung cancer. While many models demonstrate moderate to good discrimination, few are externally validated and reporting quality is variable, limiting clinical applicability and highlighting the need for robust, transparent model development ...
Evangeline Samuel, S. Tissera, E. Paul, J. Zalcberg, R. G. Stirling   +4 more
wiley   +1 more source

Extra gene coding capacity of SARS-CoV-2 provides a virus engineering platform for in vitro and in vivo applications. [PDF]

Proc Natl Acad Sci U S A
Kim T, Biswas A, Kandel S, Kuroda M, Halfmann PJ, Kawaoka Y.   +5 more
europepmc   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Efficient lentivirus concentration using a tabletop centrifuge. [PDF]

Mol Biol Rep
Müller E, Boesner J, Karaköse Z, Schwarzer R, Schwarzer-Sperber HS.   +4 more
europepmc   +1 more source

Super‐Refractory Status Epilepticus (SRSE) in a Patient With Compound Heterozygous OPA1 Variants: Case Report and Literature Review

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Super‐Refractory Status Epilepticus (SRSE) is a rare, life‐threatening neurological emergency with unclear etiology in many cases. Mitochondrial dysfunction, often due to disease‐causing genetic variants, is increasingly recognized as a cause, with each gene producing distinct pathophysiological mechanisms.
Pouria Mohammadi, Lara Basovic, Christopher Michael McGraw   +2 more
wiley   +1 more source

The 3'Untranslated region is a critical determinant of Getah virus replication, pathogenesis, and vector competence. [PDF]

Virulence
Ren T, Li P, Liu M, Zhang L, Zhong Z, Wang G, Wang X, Zhou L, Qin Y, Ouyang K, Yin Y, Chen Y, Huang W, Wei Z.   +13 more
europepmc   +1 more source