Results 121 to 130 of about 1,983,859 (250)

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

New generation sequencing and effectiveness of the influenza vaccine: a study on influenza A (H3) virus in patients with influenza, 2016/2017 and 2017/2018 [PDF]

, 2018
A tecnologia de sequenciação de nova geração (NGS) permite uma análise genética do vírus da gripe muito mais profunda, quando comparada com a sequenciação pelo método de Sanger, pois permite a análise do genoma viral completo (e não apenas do gene da ...
Borges, Vítor, Conde, Patrícia, Costa, Inês, Cristóvão, Paula, Gomes, João Paulo, Gomez, Verónica, Guiomar, Raquel, Kislaya, Irina, Machado, Ausenda, Mendonça, Joana, Nunes, Baltazar, Pechirra, Pedro, Rodrigues, Ana Paula   +12 more
core  

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Diferenças de produtividade entre cultivares de alho obtidas por cultura de tecidos e multiplicação convencional em um período de nove anos consecutivos

Bioscience Journal, 2010
O objetivo deste trabalho foi estudar a campo, o comportamento de cinco cultivares de alho proveniente de cultura de tecidos e estabelecer um tempo máximo de multiplicação convencional de cada cultivar. Os trabalhos foram conduzidos na área experimental
Ernani Clarete da Silva, Rovilson José de Souza, Moacir Pasqual   +2 more
doaj  

Suscetibilidade dos vírus da gripe aos antivirais inibidores da neuraminidase em Portugal, 2009-2014 [PDF]

, 2014
Os antivirais têm também uma longa e extensa história no tratamento e prevenção da gripe, sendo hoje uma importante opção para o tratamento da doença, bem como a medida de prevenção de eleição numa fase inicial de uma pandemia.
Conde, Patrícia, Cristóvão, Paula, Furtado, Cristina, Guiomar, Raquel, Maia, Ana Carina, Pechirra, Pedro   +5 more
core  

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Detecção por RT-PCR em Tempo Real e caracterização molecular do Grapevine virus D e Grapevine leafroll-associated virus 5 em videira. [PDF]

, 2019
O objetivo deste trabalho foi verificar a ocorrência destes dois vírus em videiras por meio de RT-PCR em Tempo Real, além de caracterizar molecularmente três dos isolados obtidos ...
DUBIELA, C. R., EIRAS, M., FAJARDO, T. V. M., NICKEL, O., REVERS, L. F., SOUTO, E. R. de   +5 more
core  

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source