Results 81 to 90 of about 759,009 (305)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Building trust and equity in vaccine communication through community engagement

Human Vaccines & Immunotherapeutics
That the COVID-19 pandemic has exacerbated inequities in health has been well studied in recent years, yet the ways in which the pandemic has also revealed existing inequities in communication, specifically health communication, is less well understood ...
Theresa Sommers, Meredith Dockery, Nina Burke, Sharin D’Souza, Brittany Troupe, Tina Agbonyinma, Harikeerthan Raghuram, Kathryn L. Hopkins, Elizabeth Kohlway, Pedja Stojicic, Anant Bhan   +10 more
doaj   +1 more source

Vaccination: Home Visits to Increase Vaccination Rates

The Community Preventive Services Task Force (CPSTF) findings and recommendations for "Increasing Appropriate Vaccination: Home Visits to Increase Vaccination Rates" are based on systematic reviews of evidence of effectiveness and economic impact.

core   +1 more source

Previous Vaccination History and Psychological Factors as Significant Predictors of Willingness to Receive Mpox Vaccination and a Favorable Attitude towards Compulsory Vaccination

, 2023
During the ongoing multi-country monkeypox (Mpox) outbreak, healthcare workers (HCWs) have represented a key group in mitigating disease spread. The current study aimed to evaluate the attitude of nurses and physicians in Jordan towards Mpox vaccination,
Razan Aburumman, Aburumman, Razan, Barakat, Muna, Muna Barakat, Alraie, Lana A.,, Al-Tammemi, Ala’A B.,, Sakher Alhiary, Ala’a B. Al-Tammemi, Aburumman, Razan,, Kholoud Al-Mahzoum, Malik Sallam, Al-Naimat, Hala, Lana A. AlRaie, Nesreen A. Salim, Haneen Mahameed, Salim, Nesreen A., Alhiary, Sakher,, Hala Al-Naimat, Alraie, Lana A., Sallam, Malik, Mahameed, Haneen, Barakat, Muna,, Alhiary, Sakher, Salim, Nesreen A.,, Sallam, Malik,, Lund University., Al-Mahzoum, Kholoud, Al-Tammemi, Ala’A B.   +27 more
core   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

The Economic Case for Expanding Vaccination Coverage of Children [PDF]

While childhood vaccination programs, such as WHO’s Expanded Program on Immunization, have had a dramatic impact on child morbidity and mortality worldwide, lack of coverage with several existing vaccines is responsible for large numbers of child deaths ...
Lois Privor-Dumm, Damian Walker, Orin Levine, Jennifer O'Brien, Abigail Friedman, Till Bärnighausen, David Canning, David Bloom   +7 more
core  

Influenza Vaccination Responses in Disabled Stroke Patients: A Single‐Center Prospective Observational Study

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective This study aimed to investigate the immunological response to influenza vaccination, the incidence and severity of influenza infection, and the side effects of the vaccination in patients with ischemic stroke. Methods This prospective observational study was conducted between 2023 and 2024 at Ramathibodi Hospital.
Achiraya Pakngao, Sureerat Suwatcharangoon, Pintip Ngamjanyapron, Kobporn Boonnak, Thanitta Suangtamai, Porpon Rotjanapan   +5 more
wiley   +1 more source