Results 71 to 80 of about 837,399 (259)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Building trust and equity in vaccine communication through community engagement

Human Vaccines & Immunotherapeutics
That the COVID-19 pandemic has exacerbated inequities in health has been well studied in recent years, yet the ways in which the pandemic has also revealed existing inequities in communication, specifically health communication, is less well understood ...
Theresa Sommers, Meredith Dockery, Nina Burke, Sharin D’Souza, Brittany Troupe, Tina Agbonyinma, Harikeerthan Raghuram, Kathryn L. Hopkins, Elizabeth Kohlway, Pedja Stojicic, Anant Bhan   +10 more
doaj   +1 more source

Vaccination-challenge study in macaques.

, 2021
(A) Cartoon depicts the Spike immunogens produced from the different DNA-based vaccines and the vaccination scheme of the 6 vaccine groups. The animals from G1-G4 were challenged 5 weeks after the last vaccination (indicated by maroon arrow).
Laurent Pessaint (11467522), Evangelos Terpos (66941), Hanne Andersen (216789), Mahesh Agarwal (2745004), Xintao Hu (722037), Dimitris Stellas (11467516), Robert Burns (1728919), George N. Pavlakis (10346856), Margherita Rosati (396218), Meletios Athanasios Dimopoulos (11467525), David J. Venzon (7478117), Alexander Wlodawer (1249), Santhi Devasundaram (4885501), Mark G. Lewis (7538024), Barbara K. Felber (8532285), Jenifer Bear (534909), Duncan Donohue (6475823), James I. Mullins (9300278), Bhabadeb Chowdhury (11467519)   +18 more
core   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

The influence of previous exposure to environmental mycobacteria on the interferon-gamma response to bacille Calmette-Guérin vaccination in southern England and northern Malawi.

, 2006
We report a large study of the effect of BCG vaccination on the in vitro 6-day whole blood interferon-gamma (IFN-gamma) response to antigens from eight species of mycobacteria among schoolchildren in south-eastern England, where bacille Calmette-Guérin ...
Crampin, A.C., Nazareth, B., Stenson, S, Branson, K, Floyd, S., Weir, RE, Branson, K., Fine, P.E.M., Sichali, L., Chaguluka, SD, Chaguluka, S.D., Donovan, L., Weir, R.E., Stanley, C., Donovan, L, Black, G.F., Nazareth, B, Stenson, S., Dockrell, H.M., Sichali, L, Crampin, AC, Dockrell, HM, Black, GF, Stanley, C, Fine, PEM, Floyd, S   +25 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Risk factors associated with the intensity of COVID-19 outbreaks in Canadian community settings: a retrospective analysis of outbreak-level surveillance data

BMC Public Health
Background The severity of COVID-19 outbreaks is disproportionate across settings (e.g., long-term care facilities (LTCF), schools) across Canada. Few studies have examined factors associated with outbreak severity to inform prevention and response.
Demy Dam, Michelle Chen, Erin E. Rees, Bethany Cheng, Lynn Sukkarieh, Erin McGill, Yasmina Tehami, Anna Bellos, Jonathan Edwin, Kaitlin Patterson   +9 more
doaj   +1 more source

Determinants of influenza non-vaccination among Canadian children: insights from a nationwide survey

Frontiers in Public Health
BackgroundTo identify determinants influencing Canadian parents’ decision not to vaccinate their children aged 6 months to 17 years against seasonal influenza.MethodsData from the 2022 Childhood COVID-19 Immunization Coverage Survey, a national survey of
Abdallah Alami, Sailly Dave, Caren Uhlik, Marwa Ebrahim, Daniel Krewski, Julie Laroche   +5 more
doaj   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source