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Archives of Virology, 1977
Extracts of vaccinia-infected HeLa cells were rendered free from infectious virus by centrifugation followed by membrane filtration and were shown to be toxic to uninfected HeLa cells in the presence of hypertonic MgSO4, used as a macromolecular uptake inducer, under conditions which did not kill control cells.
J, Wolstenholme +3 more
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Extracts of vaccinia-infected HeLa cells were rendered free from infectious virus by centrifugation followed by membrane filtration and were shown to be toxic to uninfected HeLa cells in the presence of hypertonic MgSO4, used as a macromolecular uptake inducer, under conditions which did not kill control cells.
J, Wolstenholme +3 more
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Annual Review of Microbiology, 2003
▪ Abstract Vaccinia virus (VV), the virus smallpox vaccine, replicates in the cytoplasm of infected cells. The intracellular movement of this large virus would be inefficient without specific transport mechanisms; therefore, VV uses microtubules for movement during both entry and egress.
Geoffrey L, Smith +2 more
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▪ Abstract Vaccinia virus (VV), the virus smallpox vaccine, replicates in the cytoplasm of infected cells. The intracellular movement of this large virus would be inefficient without specific transport mechanisms; therefore, VV uses microtubules for movement during both entry and egress.
Geoffrey L, Smith +2 more
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Vaccinia Virus Expression Vectors
Annual Review of Immunology, 1987Infectious diseases remain a major problem throughout the world. It is clear, at least for viruses, that prevention is our best defense. Most successful vaccines (e.g., smallpox, rubella, Sabin poliomyelitis, measles, mumps, yellow fever) have consisted of live attenuated viruses.
B, Moss, C, Flexner
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Journal of Virology, 1985
Input vaccinia virus deoxyribonucleoproteids with buoyant densities (in CsCl) very similar (if not identical) to those of viral cores have been found in large cytoplasmic structures in which viral DNA replication takes place. The deoxyribonucleoproteids consist of at least five major and two minor core proteins and viral DNA which is protected against ...
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Input vaccinia virus deoxyribonucleoproteids with buoyant densities (in CsCl) very similar (if not identical) to those of viral cores have been found in large cytoplasmic structures in which viral DNA replication takes place. The deoxyribonucleoproteids consist of at least five major and two minor core proteins and viral DNA which is protected against ...
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1989
Several viruses agglutinate erythrocytes of various animal species. The components responsible for this phenomenon have been termed hemagglutinins (HA), which in many cases have been identified as glycoproteins present in virus envelopes. The HA titers have often been used as convenient indicators of the amounts of viruses.
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Several viruses agglutinate erythrocytes of various animal species. The components responsible for this phenomenon have been termed hemagglutinins (HA), which in many cases have been identified as glycoproteins present in virus envelopes. The HA titers have often been used as convenient indicators of the amounts of viruses.
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Vaccinia virus morphogenesis and dissemination
Trends in Microbiology, 2008Vaccinia virus is the smallpox vaccine. It is the most intensively studied poxvirus, and its study has provided important insights about virus replication in general and the interactions of viruses with the host cell and immune system. Here, the entry, morphogenesis and dissemination of vaccinia virus are considered.
Kim L, Roberts, Geoffrey L, Smith
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Vaccinia Virus Expression Vectors
Journal of General Virology, 1986Introduction. Recombinant DNA technology has made possible the expression of heterologous genes in a variety of animal viruses (for review, see Rigby, 1983). The availability of a wide variety of animal virus vectors enables a foreign gene to be expressed in different cell types, at different levels and with different consequences for the host cell ...
M, Mackett, G L, Smith
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Vaccinia: Virus, Vector, Vaccine
1988Publisher Summary This chapter discusses the genetic characterization of vaccinia, a general protocol for the insertion of foreign genes into vaccinia virus, and analysis of the recombinant viruses. The vaccinia virus can infect a variety of animals and is able to replicate in a variety of tissue culture cells. When experimenting with this virus, two
A, Piccini, E, Paoletti
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Topography of vaccinia virus DNA
Virology, 1977Abstract Contour length measurements in an electron microscope of vaccinia genomes released from virions by lysis in Sarkosyl and 2-mercaptoethanol at 4° on the surface of gradients, followed by sedimentation into the gradients to remove proteins, showed that the linear, double-stranded (ds) viral DNA molecules prepared in this way, had a MW of 132 ...
M, Esteban, L, Flores, J A, Holowczak
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The wet preservation of vaccinia virus
Archiv f�r die gesamte Virusforschung, 1966Buffered glycerol was shown to be an efficient preservation medium for vaccinia virus, but under the described experimental conditions it was not superior to serum, glycerol-serum, glycerol-broth or fortified Earle's saline in respect of preservative qualities, although it was in respect of its defined chemical composition.
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