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Vaccinia Virus Expression Vectors
Annual Review of Immunology, 1987Infectious diseases remain a major problem throughout the world. It is clear, at least for viruses, that prevention is our best defense. Most successful vaccines (e.g., smallpox, rubella, Sabin poliomyelitis, measles, mumps, yellow fever) have consisted of live attenuated viruses.
B, Moss, C, Flexner
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Journal of Virology, 1985
Input vaccinia virus deoxyribonucleoproteids with buoyant densities (in CsCl) very similar (if not identical) to those of viral cores have been found in large cytoplasmic structures in which viral DNA replication takes place. The deoxyribonucleoproteids consist of at least five major and two minor core proteins and viral DNA which is protected against ...
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Input vaccinia virus deoxyribonucleoproteids with buoyant densities (in CsCl) very similar (if not identical) to those of viral cores have been found in large cytoplasmic structures in which viral DNA replication takes place. The deoxyribonucleoproteids consist of at least five major and two minor core proteins and viral DNA which is protected against ...
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1989
Several viruses agglutinate erythrocytes of various animal species. The components responsible for this phenomenon have been termed hemagglutinins (HA), which in many cases have been identified as glycoproteins present in virus envelopes. The HA titers have often been used as convenient indicators of the amounts of viruses.
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Several viruses agglutinate erythrocytes of various animal species. The components responsible for this phenomenon have been termed hemagglutinins (HA), which in many cases have been identified as glycoproteins present in virus envelopes. The HA titers have often been used as convenient indicators of the amounts of viruses.
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Topography of vaccinia virus DNA
Virology, 1977Abstract Contour length measurements in an electron microscope of vaccinia genomes released from virions by lysis in Sarkosyl and 2-mercaptoethanol at 4° on the surface of gradients, followed by sedimentation into the gradients to remove proteins, showed that the linear, double-stranded (ds) viral DNA molecules prepared in this way, had a MW of 132 ...
M, Esteban, L, Flores, J A, Holowczak
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Immunoprecipitating factors of vaccinia virus
Virology, 1965Abstract Vaccinia virus particles and soluble vaccinial antigens were separated. Preparations of soluble antigens contained nine different immunoprecipitating factors. Mechanical disintegration of vaccinia virus, freed from soluble antigens, released eight precipitinogens all of which corresponded immunologically to precipitinogens demonstrable among
J, Marquardt, S E, Holm, E, Lycke
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Vaccinia: Virus, Vector, Vaccine
1988Publisher Summary This chapter discusses the genetic characterization of vaccinia, a general protocol for the insertion of foreign genes into vaccinia virus, and analysis of the recombinant viruses. The vaccinia virus can infect a variety of animals and is able to replicate in a variety of tissue culture cells. When experimenting with this virus, two
A, Piccini, E, Paoletti
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Vaccinia Virus Expression Vectors
Journal of General Virology, 1986Introduction. Recombinant DNA technology has made possible the expression of heterologous genes in a variety of animal viruses (for review, see Rigby, 1983). The availability of a wide variety of animal virus vectors enables a foreign gene to be expressed in different cell types, at different levels and with different consequences for the host cell ...
M, Mackett, G L, Smith
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Vaccinia virus morphogenesis and dissemination
Trends in Microbiology, 2008Vaccinia virus is the smallpox vaccine. It is the most intensively studied poxvirus, and its study has provided important insights about virus replication in general and the interactions of viruses with the host cell and immune system. Here, the entry, morphogenesis and dissemination of vaccinia virus are considered.
Kim L, Roberts, Geoffrey L, Smith
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Radiation Inactivation of Vaccinia Virus
Radiation Research, 1961Radiation inactivation of P/sup 3//sup 2/-labeled vaccinia virus indicates that the virus possesses one or two sites which are responsible for attachment to the host cell. The sensitive volume of the attachment site was found to correspond to a molecular weight of about 3 million.
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The structure of vaccinia virus
The Journal of Pathology and Bacteriology, 1954G, EAVES, T H, FLEWETT
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