Results 161 to 170 of about 2,020 (179)

VapC toxin switches M. smegmatis cells into dormancy through 23S rRNA cleavage

Archives of Microbiology, 2022
Mycobacterium tuberculosis is an extremely successful pathogen known for its ability to cause latent infection. The latter is connected with the bacterium resting state development and is considered to be based on the activity of toxin-antitoxin (TA) systems at least in part.
Mikhail Zamakhaev, Artem Grigorov, Julia Bespyatykh, Tatyana Azhikina, Anna Goncharenko, Mikhail Shumkov   +5 more
openaire   +3 more sources

Structure-Based De Novo Design of Mycobacterium Tuberculosis VapC-Activating Stapled Peptides

ACS Chemical Biology, 2020
Toxin-antitoxin (TA) systems have been considered essential factors for bacterial survival. During our drug development program aimed against tuberculosis (TB), we discovered certain peptides that mimic the binding of the VapBC30 complex, leading to the arrest of bacterial cell growth and eventually cell death.
Sung-Min Kang, Heejo Moon, Sang-Woo Han, Do-Hee Kim, Byeong Moon Kim, Bong-Jin Lee   +5 more
openaire   +3 more sources

Structural analysis of the active site architecture of the VapC toxin from Shigella flexneri

Proteins: Structure, Function, and Bioinformatics, 2016
ABSTRACTThe VapC toxin from the Shigella flexneri 2a virulence plasmid pMYSH6000 belongs to the PIN domain protein family, which is characterized by a conserved fold with low amino acid sequence conservation. The toxin is a bona fide Mg2+‐dependent ribonuclease and has been shown to target initiator tRNAfMet in vivo. Here, we present crystal structures
Xu, Kehan, Dedic, Emil, Brodersen, Ditlev Egeskov   +2 more
openaire   +4 more sources

VapC toxin inhibition as a method for prevention of the formation of resting forms of mycobacteria

Applied Biochemistry and Microbiology, 2016
It has been shown that inactivation of the VapC toxin from the VapВС toxin-antitoxin system prevents mycobacterial cells from transitioning to an ovoid state that meets the criteria of dormancy. The results indicate a potential target for medicines that prevent the development of latent tuberculosis infection and provide a basis to obtain bacterial ...
O I, Demidenok, M S, Shumkov, A V, Goncharenko   +2 more
openaire   +3 more sources

Ectopic production of VapCs from Enterobacteria inhibits translation and trans‐activates YoeB mRNA interferase

Molecular Microbiology, 2009
SummaryToxin–antitoxin loci have been identified in almost all free‐living prokaryotes, often in high copy numbers. The biological function and molecular targets of the abundant vapBC loci are not yet known. Here we analyse the vapBC loci of Salmonella LT2 and Shigella plasmid pMYSH6000.
Winther KS, Gerdes K
openaire   +4 more sources

Co‐expression and purification of a toxin‐antitoxin VapC–VapB complex from the E. coli O145:H28 strain RM12581 associated with a Romain lettuce outbreak.

The FASEB Journal, 2020
VapC and VapB are the toxin and anti‐toxin proteins, respectively, of the VapCB toxin–antitoxin system found in bacteria and associated with stunting cell growth during stressful conditions. VapC homologs typically are tRNA endonucleases, and VapB homologs are known to bind to double‐stranded DNA, as well as VapC to inihibit its ...
Seth Patrick Jones, Christian Goossen, Sean Lewis, Annie Delaney, Michael Gleghorn   +4 more
openaire   +2 more sources

Integrated host–phage RNA seq analysis of Mycobacteriophage D29 infection reveals dual-arm balanced phage genome expression and inhibited expression of host transporter and VapC target genes

ABSTRACT The Mycobacteriophage D29 infects Mycobacterium species. Many of these are deadly human pathogens. In earlier studies, we performed proteomic analyses using a temperature-sensitive variant of D29, called D29ts12.
Rahul Shaw, Shrestha Ghosh, Subhajit Dutta, Anirban Ghosh, Sujoy K. Das Gupta   +4 more
openaire   +2 more sources

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Resonance assignments of a VapC family toxin from Clostridium thermocellum

Biomolecular NMR Assignments, 2016
Toxin-antitoxin (TA) systems widely exist in bacterial plasmids, phages, and chromosomes and play important roles in growth persistence and host-pathogen interaction. Virulence associated protein BC (VapBC) family TAs are the most abundant TAs in bacteria and many pathogens contain a large number of vapBC loci in the genome which have been extensively ...
Chen Wang, Jinsong Xuan, Qiu Cui, Yingang Feng   +3 more
openaire   +2 more sources

Treatment of feline intermediate to high‐grade alimentary lymphoma: A retrospective evaluation of 55 cats treated with the VAPC combination chemotherapy protocol (2017–2021)

Veterinary and Comparative Oncology, 2023
AbstractThe most commonly utilized protocols to treat lymphoma in cats employ vincristine, cyclophosphamide and prednisone; with additional drugs sometimes used including L‐asparaginase and doxorubicin. Medical records were reviewed for 55 cats with alimentary lymphoma treated with a novel multiagent protocol using prednisolone, L‐asparaginase ...
Antony Stewart Moore, Angela Erica Frimberger   +1 more
openaire   +2 more sources