Results 131 to 140 of about 2,628,537 (340)

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

Journal of Bacteriology, 1995
L. Guzman, D. Belin, M. Carson, J. Beckwith   +3 more
semanticscholar   +1 more source

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

Dissipative vector solitons in a dispersionmanaged cavity fiber laser with net positive cavity dispersion

, 2009
Han Zhang, Dingyuan Tang, Luming Zhao, Xuan Wu, Hwa‐Yaw Tam   +4 more
openalex   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

GATEWAY vectors for Agrobacterium-mediated plant transformation.

Trends in Plant Science, 2002
M. Karimi, D. Inzé, A. Depicker
semanticscholar   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Morphological traits and mitogenome of Thaumapsylla breviceps orientalis endemic to China provide insight into the evolution of the order Siphonaptera

Scientific Reports
The Thaumapsylla breviceps orientalis is endemic to China and exhibits extreme host specificity (monoxenous). This study reports the first mitogenome sequenced for the family Ischnopsyllidae and provides comprehensive analysis of both the morphological ...
Ju Pu, Xiaoxia Lin, Xiaobin Huang, Xiaoyan Zheng, Xianzheng Zhang, Wenge Dong   +5 more
doaj   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Fusion of HCV Nonstructural Antigen to MHC Class II–associated Invariant Chain Enhances T-cell Responses Induced by Vectored Vaccines in Nonhuman Primates [PDF]

, 2014
Stefania Capone, Mariarosaria Naddeo, Anna Morena D’Alise, Adele Abbate, Fabiana Grazioli, Annunziata Del Gaudio, Mariarosaria Del Sorbo, Maria Luisa Esposito, Virginia Ammendola, Gemma Perretta, Alessandra Taglioni, Stefano Colloca, Alfredo Nicosia, Riccardo Cortese, Antonella Folgori   +14 more
openalex   +1 more source