Results 71 to 80 of about 157,231 (265)

Cause of jet of vortex ring and its suppression in circular converging nozzle with entrance pipe

open access: yesNihon Kikai Gakkai ronbunshu, 2019
The control of circular jets has been studied extensively in the fluid dynamics literature. Most studies introduce an entrance pipe upstream of the nozzle outlet to straighten the flow.
Masato AKIMOTO, Motoaki KIMURA
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Flow field analysis and particle erosion of tunnel‐slope systems under coupling between runoff and fast (slow) seepage

open access: yesDeep Underground Science and Engineering
The presence of particles on the surface of a tunnel slope renders it susceptible to erosion by water flow, which is a major cause of soil and water loss.
Shuai Zhang   +5 more
doaj   +1 more source

Rheological and Flocculation Analysis of Microfibrillated Cellulose Suspension Using Optical Coherence Tomography

open access: yesApplied Sciences, 2018
A sub-micron resolution optical coherence tomography device was used together with a pipe rheometer to analyze the rheology and flocculation dynamics of a 0.5% microfibrillated cellulose (MFC) suspension.
Antti I. Koponen   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Longitudinal Mean Velocity and Turbulent Kinetic Energy Within an Emergent Canopy in Nonuniform Flows

open access: yesWater Resources Research
Vegetation‐induced drag generates nonuniform water surface profiles through flow disruption, creating complex hydrodynamic conditions characterized by enhanced turbulence and energy dissipation.
Yonggang Zhang   +4 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

open access: yesFEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain   +11 more
wiley   +1 more source

Harnessing tidal energy with breakwater-integrated marine turbines for coastal protection

open access: yesEnvironmental Research Communications
This study presents the design and evaluation of a vertical axis tidal micro-turbine system integrated into an improved wave breakwater, based on the Wave Breakwater and Coral Restorer (WABCORE) structure, which was developed through a collaborative ...
A A Rahman   +2 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

open access: yesFEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada   +7 more
wiley   +1 more source

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