Results 41 to 50 of about 72,292 (222)

UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation

Advanced Science, EarlyView.
ABSTRACT Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organized and maintained in cells remains poorly understood. Here we identify ubiquitin C‐terminal hydrolase L3 (UCHL3) as a post‐translational regulator of flavivirus sfRNA stability and function.
Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyal   +7 more
wiley   +1 more source

Electrospun Poly (ε-Caprolactone) Nanofiber Mat as Extracellular Matrix [PDF]

Cell Journal, 2008
Objective: Investigation of the suitability of electrospun Poly (ε-caprolactone) (PCL)nanofiber scaffold for the Vero cell culture.Materials and Methods: Electrospinning was used for production of PCL nanofibersscaffolds.
Laleh Ghasemi-Mobarakeh, Mohammad Morshed,, Khadije Karbalaie, Mehrafarin Fesharaki, Mohammad Hossein Nasr-Esfahani, Hossein Baharvand   +5 more
doaj  

Preparation and evaluation of a novel, live, attenuated influenza H1N1 vaccine strain produced by a modified classical reassortment method

Human Vaccines & Immunotherapeutics, 2018
Live attenuated influenza vaccine (LAIV)-based Vero cells could provide a better choice to control and prevent influenza virus infections. This study used the human influenza virus A/Yunnan/1/2005Vca(H3N2) (YN/05Vca) as a donor strain.
Yunfeng Long, Lei Ma, Ze Liu, Shaohui Song, Xingliang Geng, Fan Yang, Qi Guo, Zhuofan Li, Weidong Li, Guoyang Liao   +9 more
doaj   +1 more source

Mycoplasma detection in a mouse cell line and possible contamination source

Revista Peruana de Biología, 2014
The detection of contaminating mycoplasma in a NS-1 myeloma celI line was studied comparing three methods The probable source of mycoplasma contamination were some batches of commercial sera Fetal Bovine Sera (FBS) mouse myeloma NS-1 cells and Vero ...
Willian Cornejo M., Libertad Alzamora G.
doaj   +1 more source

New World hantaviruses activate IFNlambda production in type I IFN-deficient vero E6 cells. [PDF]

PLoS ONE, 2010
Hantaviruses indigenous to the New World are the etiologic agents of hantavirus cardiopulmonary syndrome (HCPS). These viruses induce a strong interferon-stimulated gene (ISG) response in human endothelial cells.
Joseph Prescott, Pamela Hall, Mariana Acuna-Retamar, Chunyan Ye, Marc G Wathelet, Hideki Ebihara, Heinz Feldmann, Brian Hjelle   +7 more
doaj   +1 more source

Cysteine Redox State Governs the Condensation Pathway of Hendra Virus W Protein and Differentially Impacts Type I IFN and NF‐κB Signaling

Advanced Science, EarlyView.
The cysteine redox state of the Hendra virus W (WHeV) protein controls assembly into fibrils or amorphous aggregates, with residues 2–29 required for fibrillation. In cells, WHeV forms different types of nuclear condensates. Impaired ability to form redox‐sensitive, non‐filamentous condensates reduces WHeV inhibition of the NF‐κB pathway, while it ...
Frank Gondelaud, Alexandre Lalande, Giulia Pesce, Auriane Bouchet, Carole Meunier, Christophe Bignon, Denis Ptchelkine, Yu Gu, Eva Ogire, Pierre‐Yves Lozach, Denis Gerlier, Cyrille Mathieu, Sonia Longhi   +12 more
wiley   +1 more source

Virus‐Mediated Self‐Assembly of Functional Cyclodextrins for Antiviral Inhibition

Angewandte Chemie, EarlyView.
A sialic acid (SA)‐functionalized cyclodextrin (CD) self‐assembles on SARS‐CoV‐2 through ligand–receptor recognition, nucleating cooperative supramolecular polymer growth that blocks infection. The adaptive assembly requires both ligand binding and host–guest polymerization, remains active across variants, and can be tuned by coassembly, establishing ...
Pedro J. Hernando, Laora Boulo, Léonid Lavnevich, Elisa Teyssou, Stéphane Marot, Adélie Gothland, Jean‐Michel Guigner, Anne‐Geneviève Marcelin, Mickaël Ménand, Vincent Calvez, Matthieu Sollogoub   +10 more
wiley   +2 more sources

Dengue type 4 live-attenuated vaccine viruses passaged in vero cells affect genetic stability and dengue-induced hemorrhaging in mice. [PDF]

PLoS ONE, 2011
Most live-attenuated tetravalent dengue virus vaccines in current clinical trials are produced from Vero cells. In a previous study we demonstrated that an infectious cDNA clone-derived dengue type 4 (DEN-4) virus retains higher genetic stability in MRC ...
Hsiang-Chi Lee, Yu-Ting Yen, Wen-Yu Chen, Betty A Wu-Hsieh, Suh-Chin Wu   +4 more
doaj   +1 more source

RNF138‐Mediated Ubiquitination and Degradation of NS5 Restricts Tick‐Borne Encephalitis Virus Infection

Advanced Science, EarlyView.
Host‐specific compatibility between RNF138‐like proteins and flavivirus NS5 determines NS5 stability. Mammalian RNF138 but not arthropod homologs recognizes and induces conserved NS5/RdRp K48‐linked ubiquitination and proteasomal degradation, thereby restricting viral replication. Ectopic RNF138 in mice attenuates TBEV‐induced pathogenesis. (Created in
Jialiang Sun, Weijing Yang, Hao Zhou, Shuai Li, Xin Jiang, Jianyang Gu, Wenyan Zhang   +6 more
wiley   +1 more source

Supramolecular Polymer‐Surfactant Co‐Assemblies for Multivalent HSV‐1 Inhibition

Angewandte Chemie, EarlyView.
Non‐covalent co‐assembly of BTA with antiviral surfactants produces ratio‐dependent morphologies. Fibrous supramolecular polymers exhibit superior HSV‐1 inhibition, revealing multivalent fiber‐virus interactions and highlighting supramolecular co‐assemblies as a powerful strategy for functional biomedical nanomaterials with demonstrated tunable ...
Christian Zoister, Boris Schade, Paulina Sittinger, Xianwen Lou, Esmee de Korver, Freek V. de Graaf, Ranen Etouki, Chuanxiong Nie, Kai Licha, Kai Ludwig, Abhishek Kumar Singh, E. W. Meijer, Rainer Haag   +12 more
wiley   +2 more sources