Results 21 to 30 of about 72,292 (222)

Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney

open access: yesmBio, 2016
A hepatitis C virus (HCV) cell culture system incorporating the JFH-1 strain and the human hepatoma cell line HuH-7 enabled the production of infectious HCV particles. Several host factors were identified as essential for HCV replication. Supplementation
Asako Murayama   +3 more
doaj   +1 more source

Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System [PDF]

open access: yesInternational Journal of Fertility and Sterility, 2010
Background This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality ...
Mohsen Forouzanfar   +5 more
doaj   +1 more source

Propagation of Pneumocystis carinii in Vero cell culture [PDF]

open access: yesInfection and Immunity, 1978
Pneumocystis carinii derived from infected murine lung was found to be capable of limited growth in Vero African green monkey kidney cell cultures. The observed increase in the number of cyst forms was influenced by the ratio of cysts to host cells in the inocula, duration of passage, and formulation of the cell culture media.
L L, Pifer, D, Woods, W T, Hughes
openaire   +2 more sources

Vero cells gain renal tubule markers in low-calcium and magnesium chemically defined media

open access: yesScientific Reports, 2022
In this study, a chemically defined, animal component-free media was developed to promote Vero growth in suspension. Key media compounds were screened using Plackett–Burman styled experiments to create a media formulation to support suspension growth ...
Megan Logan   +3 more
doaj   +1 more source

Identification of Critical Amino Acids of Coxsackievirus A10 Associated with Cell Tropism and Viral RNA Release during Uncoating

open access: yesViruses, 2023
Coxsackievirus A10 (CV-A10) is a prevailing causative agent of hand–foot–mouth disease, necessitating the isolation and adaptation of appropriate strains in cells allowed for human vaccine development.
Jie Pei   +8 more
doaj   +1 more source

Impact of Chitosan and Calcium phosphate nanoparticles upon VERO cells and their adapted PPRV [PDF]

open access: yesBenha Veterinary Medical Journal, 2018
Nanotechnology that created nanoparticles (NP) having superior properties compared to the original ones offers a vast era for PPR vaccine improvement. African green monkey kidney VERO cell lines were widely used for isolation and propagation of Peste des
Mohamed Saafan   +3 more
doaj   +1 more source

A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells

open access: yesnpj Vaccines, 2021
An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to ...
Simon G. P. Funnell   +16 more
doaj   +1 more source

The order of events on Avian coronavirus life cycle shapes the order of quasispecies evolution during host switches [PDF]

open access: yesGenetics and Molecular Biology, 2022
To understand the population genetics events during coronavirus host switches, the Beaudette strain of Avian coronavirus (AvCoV) adapted to BHK-21 cells was passaged 15 times in VERO cells, the virus load and the variants at each passage being determined
Paulo E. Brandão   +4 more
doaj   +1 more source

Improved Zika virus plaque assay using Vero/TMPRSS2 cell line

open access: yesMicrobiology Spectrum
Plaque assay is the gold standard for the quantification of viable cytopathic viruses like Zika virus (ZIKV). Some strains of ZIKV produce plaques that are very difficult to accurately visualize and count on the commonly used Vero cell line.
Olukunle O. Oluwasemowo   +3 more
doaj   +1 more source

Construction of eukaryotic expression vector of Interleukin-2 gene from Xiaoshan Chicken and its expression in Vero cells

open access: yes浙江大学学报. 农业与生命科学版, 2003
In previous study IL-2 gene of Xiaoshan Chicken has been cloned by our laboratory, and in this study the cDNA of IL-2 was amplified and inserted into the eukaryotic expression vector pCI.
XIE Rong-hui   +4 more
doaj   +1 more source

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