NPC1 KO validation in Vero cells.
(A) NPC1 detection in Vero or Vero NPC1 KO cell lines (B) Indirect immunofluorescence shows NPC1 in green detected with a specific antibody against NPC1. Scale bar: 25 μm (C-D) NPC1 KO cells depicts dilated endosomes detected in red with the acidic probe
Fátima Lasala (394781) +11 more
core +1 more source
Vaccination is the practiced and accessible measure for preventing influenza infection. Because chicken embryos used for vaccine production have various insufficiencies, more efficient methods are needed.
Jian Zhou +6 more
doaj +1 more source
New World hantaviruses activate IFNlambda production in type I IFN-deficient vero E6 cells. [PDF]
Hantaviruses indigenous to the New World are the etiologic agents of hantavirus cardiopulmonary syndrome (HCPS). These viruses induce a strong interferon-stimulated gene (ISG) response in human endothelial cells.
Joseph Prescott +7 more
doaj +1 more source
Parainfluenza virus 5 genomes are located in viral cytoplasmic bodies whilst the virus dismantles the interferon-induced antiviral state of cells [PDF]
This work was supported by the Wellcome Trust.Although the replication cycle of parainfluenza virus type 5 (PIV5) is initially severely impaired in cells in an interferon (IFN)-induced antiviral state, the virus still targets STAT1 for degradation.
Carlos, T S +4 more
core +1 more source
SENSITIVITY OF SOME CELL LINES TO ADAPT PESTE DES PETITS RUMINANTS’ VIRUS FOR VACCINE PRODUCTION [PDF]
Preparation of peste des petits ruminants virus (PPRV) vaccine is traditionally carried out by adapted virus in Vero cells with typical cytopathic effects (CPEs).
MOHAMED KHODEIR +6 more
doaj +1 more source
Dengue type 4 live-attenuated vaccine viruses passaged in vero cells affect genetic stability and dengue-induced hemorrhaging in mice. [PDF]
Most live-attenuated tetravalent dengue virus vaccines in current clinical trials are produced from Vero cells. In a previous study we demonstrated that an infectious cDNA clone-derived dengue type 4 (DEN-4) virus retains higher genetic stability in MRC ...
Hsiang-Chi Lee +4 more
doaj +1 more source
Vero cells were incubated with purified Stx2 (1 CD50) or transfected with pStx2 plasmid. After 48 h, cells were stained with Crystal Violet and analyzed by optical microscopy. Representative pictures using 200X original magnification are shown. A.
Martín Isturiz (381377) +9 more
core +1 more source
Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source
Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition
Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors ...
Diena Delaiah +9 more
doaj +1 more source
Mycoplasma detection in a mouse cell line and possible contamination source
The detection of contaminating mycoplasma in a NS-1 myeloma celI line was studied comparing three methods The probable source of mycoplasma contamination were some batches of commercial sera Fetal Bovine Sera (FBS) mouse myeloma NS-1 cells and Vero ...
Willian Cornejo M., Libertad Alzamora G.
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