Results 101 to 110 of about 466,204 (299)

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

SARS-CoV-2 Viral Load in Stool and Nasopharyngeal/Oropharyngeal Samples: Implications for Clinical Progression in Severe COVID-19 Patients

International Journal of Microbiology
The SARS-CoV-2 RNA load in different specimens has been analyzed to evaluate its correlation with disease outcomes and other factors. However, conflicting results have emerged due to variations in study design.
Mariane Vedovatti Monfardini, Brena Ramos Athaydes, Priscila Marinho Abreu, Fernanda Laís Lima Fonseca, Roberta Ferreira Ventura Mendes, Juliana Couto-Vieira, Frederico Firme Figueira, Priscilla Aquino Martins, Regina Keller, Sandra Lucia Ventorin von Zeidler, Liliana Cruz Spano   +10 more
doaj   +1 more source

Viral loads in clinical specimens and SARS manifestations. [PDF]

, 2009
1. A high viral load in nasopharyngeal aspirate (with or without a high viral load in serum) is a useful prognostic indicator of respiratory failure or mortality. The presence of viral RNA in multiple body sites is also indicative of poor prognosis.
Hung, IF, Lau, SK, Woo, PC, Yuen, KY
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Costs of streamlined HIV care delivery in rural Ugandan and Kenyan clinics in the SEARCH Studys. [PDF]

, 2018
OBJECTIVES/DESIGN:As antiretroviral therapy (ART) rapidly expands in sub-Saharan Africa using new efficient care models, data on costs of these approaches are lacking. We examined costs of a streamlined HIV care delivery model within a large HIV test-and-
Aine, Ronald, Assurah, Elly, Ayieko, James, Balzer, Laura, Black, Douglas, Brown, Lillian B, Bukusi, Elizabeth A, Charlebois, Edwin D, Clark, Tamara D, Cohen, Craig R, Havlir, Diane V, Jain, Vivek, Kamya, Moses R, Kwarisiima, Dalsone, Luo, Alex, Mwai, Daniel, Mwangwa, Florence, Mwebaza, Betty, Osmand, Thomas, Owaraganise, Asiphas, Petersen, Maya L, Shade, Starley B, Thirumurthy, Harsha   +22 more
core  

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Viral genetic variation accounts for a third of variability in HIV-1 set-point viral load in Europe [PDF]

, 2017
HIV-1 set-point viral load—the approximately stable value of viraemia in the first years of chronic infection—is a strong predictor of clinical outcome and is highly variable across infected individuals.
Albert, J, Bakker, M, Bannert, N, Berkhout, B, Bezemer, D, Blanquart, F, Cornelissen, M, Fellay, J, Fransen, K, Fraser, C, Gall, A, Gourlay, AJ, Grabowski, MK, Gunsenheimer-Bartmeyer, B, Günthard, HF, Hall, M, Hillebregt, M, Kellam, P, Kivelä, P, Kouyos, R, Laeyendecker, O, Liitsola, K, Meyer, L, Ong, SH, Porter, K, Reiss, P, Ristola, M, van Sighem, A, Vanham, G, Wymant, C   +29 more
core  

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source