Results 191 to 200 of about 254,896 (222)
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Archives of Virology, 2009
Bovine viral diarrhea virus (BVDV) nonstructural protein 5A (NS5A) is one the least studied of the BVDV proteins. Therefore, to develop a tool for unraveling the functions performed by BVDV NS5A, monoclonal antibodies (MAbs) were generated by fusion of myeloma cells with spleen cells from mice immunized with recombinant E.
Muhammad Atif, Zahoor +6 more
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Bovine viral diarrhea virus (BVDV) nonstructural protein 5A (NS5A) is one the least studied of the BVDV proteins. Therefore, to develop a tool for unraveling the functions performed by BVDV NS5A, monoclonal antibodies (MAbs) were generated by fusion of myeloma cells with spleen cells from mice immunized with recombinant E.
Muhammad Atif, Zahoor +6 more
openaire +2 more sources
Biochemical and Biophysical Research Communications, 2016
Hepatitis C virus (HCV) nonstructural protein 5B (NS5B) functions as an RNA-dependent RNA polymerase in the HCV replication complex derived from the endoplasmic reticulum in hepatic cells. In this study, NS5B was used as bait in a yeast two-hybrid assay to screen a human liver cDNA library.
Sheng-Li, Zhu +9 more
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Hepatitis C virus (HCV) nonstructural protein 5B (NS5B) functions as an RNA-dependent RNA polymerase in the HCV replication complex derived from the endoplasmic reticulum in hepatic cells. In this study, NS5B was used as bait in a yeast two-hybrid assay to screen a human liver cDNA library.
Sheng-Li, Zhu +9 more
openaire +2 more sources
[Comparison of Dengue viral nonstructural protein 1 antigen testing kits].
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine], 2018Objective: To investigate the sensitivity and specificity of commercial nonstructural protein 1 (NS1) testing kits for Dengue fever diagnose, and provide the evidence for diagnostic criteria revision. Methods: 300 PCR or virus isolation positive blood samples for dengue virus were collected from sentinel hospitals for dengue surveillance in Guangzhou ...
D, Wu +8 more
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Journal of Medical Virology, 2019
AbstractClassical swine fever virus (CSFV) is a single‐stranded RNA flavivirus that can cause serious diseases in porcine species, including symptoms of infarction, systemic hemorrhage, high fever, or depression. Viperin is an important interferon‐inducible antiviral gene that has been shown to inhibit CSFV, but the exact mechanisms by which it is able
Chunmei Xu +15 more
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AbstractClassical swine fever virus (CSFV) is a single‐stranded RNA flavivirus that can cause serious diseases in porcine species, including symptoms of infarction, systemic hemorrhage, high fever, or depression. Viperin is an important interferon‐inducible antiviral gene that has been shown to inhibit CSFV, but the exact mechanisms by which it is able
Chunmei Xu +15 more
openaire +2 more sources
The Journal of Infectious Diseases, 1992
Subgenomic hepatitis A virus (HAV) RNA sequences were translated in vitro to produce proteins representing the structural (P1) and nonstructural (P2 and P3) domains of the viral polyprotein. These proteins were used as antigens to detect the presence of antibodies in sera from acute and convalescent humans and an experimentally infected chimpanzee. All
X Y, Jia, D F, Summers, E, Ehrenfeld
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Subgenomic hepatitis A virus (HAV) RNA sequences were translated in vitro to produce proteins representing the structural (P1) and nonstructural (P2 and P3) domains of the viral polyprotein. These proteins were used as antigens to detect the presence of antibodies in sera from acute and convalescent humans and an experimentally infected chimpanzee. All
X Y, Jia, D F, Summers, E, Ehrenfeld
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1990
Mouse hepatitis virus, strain A59 cDNAs were inserted into the procaryotic fusion vector pGE374. RecA/viral/LacZ tripartite fusion proteins were synthesized from these plasmids and purified from E. coli. Antisera were raised in rabbits against these fusion proteins.
P W, Zoltick +4 more
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Mouse hepatitis virus, strain A59 cDNAs were inserted into the procaryotic fusion vector pGE374. RecA/viral/LacZ tripartite fusion proteins were synthesized from these plasmids and purified from E. coli. Antisera were raised in rabbits against these fusion proteins.
P W, Zoltick +4 more
openaire +2 more sources
Molecular characterization of rubella virus nonstructural proteins and viral RNA synthesis
2009Rubella virus (RV) is a single-strand positive RNA virus. Its 9762-nucleotide (nt) genomic RNA contains two large open readings frames (ORFs) at the 5'- and 3'-termini, encoding nonstructural and structural proteins, respectively. The nonstructural proteins (NSPs) are first translated as a 200-kDa polyprotein (p200), which undergoes a single ...
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SARS-CoV-2 viral load and shedding kinetics
Nature Reviews Microbiology, 2022Olha Puhach +2 more
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Antibodies to combat viral infections: development strategies and progress
Nature Reviews Drug Discovery, 2022Giuseppe Pantaleo +2 more
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Viral and host heterogeneity and their effects on the viral life cycle
Nature Reviews Microbiology, 2020Jennifer E Jones +2 more
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