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Viral nucleocapsid melting to measure protein: RNA interactions
Biochemical and Biophysical Research Communications, 1980Abstract Thermal denaturation of nucleocapsids of wild type (WT) vesicular stomatitis virus (VSV), containing only the nucleocapsid protein (N) and viral RNA, caused a “melting” that resulted in an A260nm absorbance increase of 140%. The nucleocapsids of two temperature-sensitive ( ts ) VSV mutants, ts G31BP and ts G22, both underwent
J V, Hughes, T C, Johnson
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In situ processing of a retroviral nucleocapsid protein by the viral proteinase
"Protein Engineering, Design and Selection", 1991The proteolytic processing pathway of the nucleocapsid protein (NC) by the viral proteinase within intact capsids of equine infectious anemia virus (EIAV) is presented. The cleavage sites are located at the carboxyl side of the first cysteine residue within the zinc-finger domains.
M M, Roberts, T D, Copeland, S, Oroszlan
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Targeting the Viral Nucleocapsid Protein in Anti-HIV-1 Therapy
Mini-Reviews in Medicinal Chemistry, 2008The nucleocapsid protein (NC) plays seminal roles in HIV replication, thus representing a major drug target. NC functions rely on its two zinc-fingers and flanking basic residues. Zinc ejectors inhibit NC functions, but with limited specificity. New classes of molecules competing with NC or its viral nucleic acid and enzyme partners are reviewed here.
de Rocquigny, H. +6 more
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CONFIRMATION OF VIRAL NUCLEOCAPSID PROTEIN PRODUCED IN RECOMBINANT ESCHERICHIA COLI CELLS
X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023: Cб. тез., 2023The N protein of orthohantaviruses is the most abundant viral protein synthesized early after infection and plays a crucial role in intracellular transportation and assembly of mature virions. The N protein was successfully produced and confirmed by SDS-PAGE and Western blotting, which is a crucial step in the sequences of procedures to develop ...
N.I. Andreeva +7 more
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Localization of proteins on viral nucleocapsids using immunoelectron microscopy
Journal of Electron Microscopy Technique, 1985AbstractWe have described an indirect immunoelectron microscope method to localize individual proteins on viral nucleocapsids using monoclonal antibodies and colloidal gold‐conjugated second antibodies. The procedure provides good binding and retention of antibodies, good resolution(< 24nm), and negligible nonspecific binding of antibodies to the ...
K. G. Murti +3 more
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Journal of Molecular Biology, 2002
All lentiviruses and oncoretroviruses examined so far encode a major nucleic-acid binding protein (nucleocapsid or NC* protein), approximately 2500 molecules of which coat the dimeric RNA genome. Studies on HIV-1 and MoMuLV using in vitro model systems and in vivo have shown that NC protein is required to chaperone viral RNA dimerization and packaging ...
Witold Surewicz +2 more
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All lentiviruses and oncoretroviruses examined so far encode a major nucleic-acid binding protein (nucleocapsid or NC* protein), approximately 2500 molecules of which coat the dimeric RNA genome. Studies on HIV-1 and MoMuLV using in vitro model systems and in vivo have shown that NC protein is required to chaperone viral RNA dimerization and packaging ...
Witold Surewicz +2 more
exaly +5 more sources
SELF-ASSEMBLY OF NUCLEOCAPSID PROTEIN WITH VIRAL RNA ON MEMBRANE SURFACES
2022The SARS-CoV-2 virus has caused the ongoing global COVID-19 pandemic. One of its structural proteins, the Nucleocapsid Protein (N Protein) that is essential for packaging the virus's RNA and facilitating the virus's replication cycle, was shown to undergo Liquid-liquid Phase Separation (LLPS) in combination with subgenomic fragments of the viral RNA ...
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Virus Genes, 1997
Hantavirus nucleocapsid protein has recently been shown to be an immunodominant antigen in hemorrhagic with renal syndrome (HFRS) inducing an early and long-lasting immune response. Recombinant proteins representing various regions of the nucleocapsid proteins as well as segments of the G1 and the G2 glycoproteins of hantavirus strains CG18-20 (Puumala
P, Gött +3 more
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Hantavirus nucleocapsid protein has recently been shown to be an immunodominant antigen in hemorrhagic with renal syndrome (HFRS) inducing an early and long-lasting immune response. Recombinant proteins representing various regions of the nucleocapsid proteins as well as segments of the G1 and the G2 glycoproteins of hantavirus strains CG18-20 (Puumala
P, Gött +3 more
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Separate domains of sendai virus P protein are required for binding to viral nucleocapsids
Virology, 1990The role of Sendai virus P protein in viral RNA synthesis involves association with the nucleocapsid template. There is evidence that the carboxyl-terminal region of P protein is responsible for this association (K. W. Ryan and D. W. Kingsbury, 1988, Virology 167, 106-112).
K W, Ryan, A, Portner
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Nucleocapsid assembly drives Ebola viral factory maturation and dispersion
CellReplication and genome encapsidation of many negative-sense RNA viruses take place in virus-induced membraneless organelles termed viral factories (VFs).
Sophie L Winter +2 more
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