Results 121 to 130 of about 1,219,846 (287)

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Suppression of NF-κB Activity: A Viral Immune Evasion Mechanism [PDF]

, 2018
Nuclear factor-κB (NF-κB) is an important transcription factor that induces the expression of antiviral genes and viral genes. NF-κB activation needs the activation of NF-κB upstream molecules, which include receptors, adaptor proteins, NF-κB (IκB ...
Qiurui Zeng, Ling Zhang, Ying Wu, Anchun Cheng, Yanling Yu, Shaqiu Zhang, Xiaoyue Chen, Mafeng Liu, Liyao Deng, Mingshu Wang, Qiao Yang, Yunya Liu, Renyong Jia, Shun Chen, Dekang Zhu, Xinxin Zhao   +15 more
core   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals [PDF]

Treatment options for viral infections are limited and viruses have proven adept at evolving resistance to many existing therapies, highlighting a significant vulnerability in our defenses.
Peter Stoilov, Mangos, Maria, Choudhary Ahmed, Martha Brown, Leng, Haomin, Chabot, Benoit, Toutant, Johanne, Filomena Grosso, Ahmed, Choudhary, Shkreta, Lulzim, Dahal, Subha, Grosso, Filomena, Johanne Toutant, Alan Cochrane, Cochrane, Alan, Subha Dahal, Haomin Leng, Benoit Chabot, Stoilov, Peter, Huesca, Mario, Ali Zahedi Amiri, Lulzim Shkreta, Mario Huesca, Attisano, Liliana, Zahedi Amiri, Ali, Liliana Attisano, Brown, Martha, Maria Mangos   +27 more
core   +1 more source

Rapid enzymatic test for phenotypic HIV protease drug resistance [PDF]

, 2003
A phenotypic resistance test based on recombinant expression of the active HIV protease in E. coli from patient blood samples was developed. The protease is purified in a rapid onestep procedure as active enzyme and tested for inhibition by five selected
Machleidt, Werner, Hoffmann, D., Assfalg-Machleidt, Irmgard, Helm, K. von der, Nitschko, H., Koszinowski, U.   +5 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

An antiviral mechanism of nitric oxide: inhibition of a viral protease.

Immunity, 1999
Although nitric oxide (NO) kills or inhibits the replication of a variety of intracellular pathogens, the antimicrobial mechanisms of NO are unknown. Here, we identify a viral protease as a target of NO. The life cycle of many viruses depends upon viral proteases that cleave viral polyproteins into individual polypeptides.
Saura, Marta, Zaragoza, Carlos, McMillan, Audrey, Quick, Richard A, Hohenadl, Christine, Lowenstein, John M, Lowenstein, Charles J   +6 more
openaire   +2 more sources

Comparison of inhibitor binding in HIV-1 protease and in non-viral aspartic proteases: the role of the flap [PDF]

, 1990
The crystal structure of HIV-1 protease with an inhibitor has been compared with the structures of non-viral aspartic proteases complexed with inhibitors.
Irene T. Weber, Weber, Irene T., Gustchina, Alla, Alla Gustchina   +3 more
core   +1 more source

Crimean-Congo Hemorrhagic Fever Virus Suppresses Innate Immune Responses via a Ubiquitin and ISG15 Specific Protease

Cell Reports, 2017
Antiviral responses are regulated by conjugation of ubiquitin (Ub) and interferon-stimulated gene 15 (ISG15) to proteins. Certain classes of viruses encode Ub- or ISG15-specific proteases belonging to the ovarian tumor (OTU) superfamily.
Florine E.M. Scholte, Marko Zivcec, John V. Dzimianski, Michelle K. Deaton, Jessica R. Spengler, Stephen R. Welch, Stuart T. Nichol, Scott D. Pegan, Christina F. Spiropoulou, Éric Bergeron   +9 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source