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Alphavirus Hybrid Virion Vaccines
1996Many members of the alphavirus family are important human or veterinary pathogens. These viruses are extremely similar in molecular architecture yet differ in host range and in the pathological consequences of infection. Despite marked structural conservation, the various species are inefficient in eliciting immunological cross protection. Previously(l)
A, Shafferman +10 more
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Virion-associated protein kinases
Cellular and Molecular Life Sciences CMLS, 2002Many purified virions, particularly enveloped virus particles (such as retrovirus, hepadnavirus, herpesvirus, orthomyxovirus, and paramyxovirus), contain protein kinase (PK) activity. This type of PK has been called virion-associated protein kinase (VAPK).
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Polyadenylate Polymerase from Vaccinia Virions
Nature New Biology, 1973HETEROGENEOUS nuclear, messenger1–9 and viral1,10–16 RNAs from eukaryotic organisms contain long polyadenylate (poly (A)) sequences of unknown function. The poly (A) is located at the 3′ terminus17–21 and may be added after transcription is completed11,22.
B, Moss, E N, Rosenblum, E, Paoletti
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Defective virions of human cytomegalovirus
Virology, 1979Abstract Passage of human cytomegalovirus at high multiplicity of infection generated defective virus particles which banded in CsCI at a lower buoyant density than standard virus. The DNA from defective virions banded at a lower buoyant density in CsCl than standard DNA and sedimented in sucrose gradients similar to standard DNA.
M L, Ramirez +3 more
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Agarose isoelectrofocusing of intact virions
Journal of Virological Methods, 1984A convenient and accurate method for determining the isoelectric points of intact virions is described. Tritium-labeled poliovirus 1 (strains Brunhilde and LSc-2) and echovirus 1 (isolates V239, V248, V212, R115 and 4CH-1) were successfully focused into sharp bands at their respective isoelectric points using a thin-layer agarose isoelectric focusing ...
K S, Zerda, C P, Gerba
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Betaherpesvirus Virion Assembly and Egress
2018Virions are the vehicle for cell-to-cell and host-to-host transmission of viruses. Virions need to be assembled reliably and efficiently, be released from infected cells, survive in the extracellular environment during transmission, recognize and then trigger entry of appropriate target cells, and disassemble in an orderly manner during initiation of a
William L, Close +2 more
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Purification of Influenza Virions
This chapter describes basic workflows for concentrating and for purifying influenza virions. It presents several ways in which ultracentrifugation can be used to concentrate influenza virions from the growth media of infected cells, with notes on the different degrees of purity that can be expected when using different approaches. These approaches areJack C, Hirst, Edward C, Hutchinson
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A rainbow of influenza virions
Nature Reviews Microbiology, 2018This study investigates the phenotypic heterogeneity in influenza virus size and composition.
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Cellular proteins in HIV virions
Reviews in Medical Virology, 1997Human immunodeficiency virus type-1 (HIV-1) virions contain both virus-encoded and cellular proteins. Recent advances in the detection, isolation, and functional characterisation of host proteins incorporated in the virion have begun to provide for new perspectives on the interactions between virus and cell.
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