Results 51 to 60 of about 146,116 (296)

Apolipoprotein E mediates evasion from hepatitis C virus−neutralizing antibodies [PDF]

, 2016
Background & Aims Efforts to develop an effective vaccine against hepatitis C virus (HCV) have been hindered by the propensity of the virus to evade host immune responses. HCV particles in serum and in cell culture associate with lipoproteins, which
Bartenschlager, Ralf, Baumert, Thomas F., Crouchet, Emilie, Fauvelle, Catherine, Felmlee, Daniel J., Fofana, Isabel, Foung, Steven K.H., Habersetzer, François, Heydmann, Laura, Hiet, Marie-Sophie, Lee, JiYoung, Lefèvre, Mathieu, Magri, Andrea, Meuleman, Philip, Milne, Ross, Patel, Arvind H., Schuster, Catherine, Vercauteren, Koen, Zeisel, Mirjam B.   +18 more
core   +3 more sources

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Catch, Cut, or Block? Versatile 4‐N‐Derivatized Sialyl Glycosides for Influenza Virus Neuraminidase Detection and Purification

Angewandte Chemie, EarlyView.
Sialyl glycosides containing 4‐N‐derivatized sialic acids synthesized by a highly efficient one‐pot two‐enzyme (OP2E) chemoenzymatic sialylation strategy have been shown to be versatile probes. They can resist sialidase cleavage, be selective substrates by influenza sialidases, or be nanomolar substrate analog‐based inhibitors and affinity ligands ...
Yue Yuan, Hai Yu, Anand Kumar Agrahari, Jin Gao, Hyeog Kang, Robert Daniels, Xi Chen   +6 more
wiley   +2 more sources

Phenotypic mixing and hiding may contribute to memory in viral quasispecies [PDF]

, 2003
Background. In a number of recent experiments with food-and-mouth disease virus, a deleterious mutant, was found to avoid extinction and remain in the population for long periods of time. This observation was called quasispecies memory.
Novella, Isabel S., Wilke, Claus O.
core   +4 more sources

Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection [PDF]

Open Biology, 2017
Successful generation of virions from infected cells is a complex process requiring orchestrated regulation of host and viral genes. Cells infected with human cytomegalovirus (HCMV) undergo a dramatic reorganization of membrane organelles resulting in ...
Jonathan Pavelin, Dominique McCormick, Stephen Chiweshe, Saranya Ramachandran, Yao-Tang Lin, Finn Grey   +5 more
doaj   +1 more source

Insights into the Dynamics and Binding Mechanisms of the Alkhumra Virus NS2B/NS3 Protease: A Molecular Dynamics Study

Advanced Theory and Simulations, EarlyView.
The ΔRMSF$\Delta {\rm RMSF}$ analysis reveals significant flexibility differences between free NS3 and the NS2B/NS3 complex, with notable deviations in specific regions. Key residues driving NS2B binding are identified, and the protonation state of catalytic serine affects oxyanion hole formation.
Jurica Novak, Shivananda Kandagalla, Ramesh Sistla   +2 more
wiley   +1 more source

DNA-Packing Portal and Capsid-Associated Tegument Complexes in the Tumor Herpesvirus KSHV. [PDF]

, 2019
Assembly of Kaposi's sarcoma-associated herpesvirus (KSHV) begins at a bacteriophage-like portal complex that nucleates formation of an icosahedral capsid with capsid-associated tegument complexes (CATCs) and facilitates translocation of an ∼150-kb dsDNA
Bi, Guo-Qiang, Dai, Xinghong, Gong, Danyang, Jih, Jonathan, Liu, Yun-Tao, Sun, Ren, Zhou, Z Hong   +6 more
core  

Adducin‐1 Facilitates Influenza Virus Endosomal Trafficking and Uncoating by Regulating Branched Actin Dynamics and Myosin IIB Activity

Advanced Science, EarlyView.
In this study, a novel mechanism is unveiled by which ADD1, acting as a molecular switch, coordinates actin branch dynamics and the transport of endocytic viruses and cargoes. Phosphorylation of ADD1 at Ser726 reduces actin branch density, enhancing endosome fusion and attachment to microtubules.
Meijun Jiang, Jiahui Zou, Yaoming Jin, Chenjun Jiang, Shaoyu Tu, Tong Chen, Jinli Guo, Yanqing Cheng, Meilin Jin, Huanchun Chen, Hongbo Zhou   +10 more
wiley   +1 more source

Unlocking the M13 (F1) virion : investigation into the role of pIII C domain of F specific filamentous bacteriophage in infection : a thesis presented in partial fulfillment of the requirements for the degree of Master of Science in Biochemistry at Massey University, Palmerston North, New Zealand [PDF]

, 2005
Filamentous phage adsorb to the host cells by binding of the N2 domain of pIII to the tip of the F pilus. Binding of N1 domain of pIII to the secondary receptor (Tol A), triggers the opening of the virion by a poorly understood mechanism.
Bennett, Nicholas James
core  

Diversity and function of phage encoded depolymerases [PDF]

, 2020
Bacteriophages of the Podoviridae family often exhibit so-called depolymerases as structural components of the virion. These enzymes appear as tail spike proteins (TSPs).
Fieseler, Lars, Knecht, Leandra E., Veljkovic, Marjan   +2 more
core   +1 more source