Results 51 to 60 of about 14,063 (258)
Highly efficient construction of infectious viroid-derived clones
Plant Methods, 2019 Background Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low
Joan Marquez-Molins +3 more
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A universal oligonucleotide microarray with a minimal number of probes for the detection and identification of viroids at the genus level. [PDF]
PLoS ONE, 2013 A major challenge in the agricultural industry is the development of techniques that can screen plant samples for viroid infection. Microarrays are promising in this regard, as their high throughput nature can potentially allow for the detection of a ...
Yongjiang Zhang +12 more
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Application of RNA silencing to plant disease resistance [PDF]
, 2012 To reduce the losses caused by plant pathogens, plant biologists have adopted numerous methods to engineer resistant plants. Among them, RNA silencing-based resistance has been a powerful tool that has been used to engineer resistant crops during the ...
Cheng-Guo Duan +2 more
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Salicylic acid: a key natural foundation for next‐generation plant defense stimulators
Pest Management Science, EarlyView.Salicylic acid is emerging as a key natural molecule in sustainable crop protection, supporting diverse strategies to stimulate and sustain plant immunity for a greener agricultural future. Abstract The field of crop protection is undergoing a major transition.
Ruth Oussou +3 more
wiley +1 more source
Marine Drugs, 2015 The effect of carrageenans on tomato chlorotic dwarf viroid (TCDVd) replication and symptom expression was studied. Three-week-old tomato plants were spray-treated with iota(ɩ)-, lambda(λ)-, and kappa(κ)-carrageenan at 1 g·L−1 and inoculated with TCDVd ...
Jatinder S. Sangha +6 more
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Plants, 2021 Viroids are small, non-coding, parasitic RNAs that promote developmental distortions in sensitive plants. We analyzed pollen of Nicotiana benthamiana after infection and/or ectopic transformation with cDNAs of citrus bark cracking viroid (CBCVd), apple ...
Lenka Steinbachová +5 more
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Adverse effects of grapevine trunk diseases on canes produced from infected rootstock mother blocks
Pest Management Science, EarlyView.High incidences of GTD pathogens in rootstock mother vine heads, with Phaeomoniella chlamydospora and Diplodia seriata affecting cane physiology and quality. One‐year‐old canes harbor systemic inoculum, highlighting the need for careful monitoring and management.
Wynand Jacobus van Jaarsveld +5 more
wiley +1 more source
Viruses, 2022 High-throughput sequencing (HTS) of host plant small RNA (sRNA) is a popular approach for plant virus and viroid detection. The major bottlenecks for implementing this approach in routine virus screening of plants in quarantine include lack of ...
Ruvini V. Lelwala +10 more
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Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian +6 more
wiley +1 more source
Viroids and Viroid-like Circular RNAs: Do They Descend from Primordial Replicators?
Life, 2022 Viroids are a unique class of plant pathogens that consist of small circular RNA molecules, between 220 and 450 nucleotides in size. Viroids encode no proteins and are the smallest known infectious agents.
Benjamin D. Lee, Eugene V. Koonin
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