Results 111 to 120 of about 497,332 (389)

Single-Cell Genomics for Virology

Viruses, 2016
Single-cell sequencing technologies, i.e., single cell analysis followed by deep sequencing investigate cellular heterogeneity in many biological settings. It was only in the past year that single-cell sequencing analyses has been applied in the field of
A. Ciuffi, Sylvie Rato, A. Telenti
semanticscholar   +1 more source

The tau isoform 1N4R confers vulnerability of MAPT knockout human iPSC‐derived neurons to amyloid beta and phosphorylated tau‐induced neuronal dysfunction

Alzheimer's &Dementia, EarlyView.
Abstract INTRODUCTION Human tau protein, composed of six brain‐specific isoforms, is a major driver of Alzheimer's disease (AD). The role of its isoforms however remains unclear and human AD models are scarce. METHODS We generated human MAPT– (tau–) knockout (KO) induced pluripotent stem cells (iPSC) using CRISPR/Cas9, differentiated these into ...
Sarah Buchholz, Mohamed Aghyad Al Kabbani, Michael Bell‐Simons, Lena Kluge, Cagla Cagmak, Jennifer Klimek, Natja Haag, Lukas C. Iohan, Audrey Coulon, Marcos R. Costa, Devrim Kilinc, Hans Zempel   +11 more
wiley   +1 more source

Automated fluorescence gating and size determination reduce variation in measured concentration of extracellular vesicles by flow cytometry

Cytometry Part A, Volume 101, Issue 12, Page 1049-1056, December 2022., 2022
Abstract Extracellular vesicles (EVs) are an upcoming biomarker for disease. However, the measured concentrations of EVs by flow cytometry are incomparable due to analytical variables. This study aimed to investigate how the choice of fluorophore, and thereby brightness, affects the measured concentration of EVs.
Angela Adriana Francisca Gankema, Bo Li, Rienk Nieuwland, Edwin van der Pol   +3 more
wiley   +1 more source

Identification and differential usage of a host metalloproteinase entry pathway by SARS-CoV-2 Delta and Omicron

iScience, 2022
Summary: The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike glycoprotein (S) binds to angiotensin-converting enzyme 2 (ACE2) to mediate membrane fusion via two distinct pathways: 1) a surface, serine protease-dependent or 2) an ...
Mehdi Benlarbi, Geneviève Laroche, Corby Fink, Kathy Fu, Rory P. Mulloy, Alexandra Phan, Ardeshir Ariana, Corina M. Stewart, Jérémie Prévost, Guillaume Beaudoin-Bussières, Redaet Daniel, Yuxia Bo, Omar El Ferri, Julien Yockell-Lelièvre, William L. Stanford, Patrick M. Giguère, Samira Mubareka, Andrés Finzi, Gregory A. Dekaban, Jimmy D. Dikeakos, Marceline Côté   +20 more
doaj  

Futuristic methods in virus genome evolution using the Third-Generation DNA sequencing and artificial neural networks [PDF]

arXiv, 2019
The Third-Generation in DNA sequencing has emerged in the last few years using new technologies that allow the production of long-read sequences. Applications of the Third-Generation sequencing enable real-time and on-site data production, changing the research paradigms in environmental and medical sampling in virology. To take full advantage of large-
arxiv  

HCV Virology

, 2021
HCV was discovered and its sequence cloned in 1989, the acme of the pioneering work of Michael Houghton's group (Choo et al., 1989). Since then, studies of the HCV replication cycle have long been hampered by the following facts : (i) HCV is a strictly human pathogen that causes a chronic infection; (ii) the target cells of HCV replication are solely ...
Pécheur, Eve, Zoulim, Fabien, Bartosch, Birke   +2 more
openaire   +2 more sources

Principles And Practice Of Clinical Virology

, 2016
Thank you very much for downloading principles and practice of clinical virology. Maybe you have knowledge that, people have search numerous times for their chosen books like this principles and practice of clinical virology, but end up in infectious ...
T. Schmitz
semanticscholar   +1 more source

Paediatric Virology: A rapidly increasing educational challenge

Experimental and Therapeutic Medicine, 2016
The ‘2nd Workshop on Paediatric Virology’, which took place on Saturday the 8th of October 2016 in Athens, Greece, provided an overview on recent views and advances on Paediatric Virology. Emphasis was given to HIV-1 management in Greece, a country under
I. Mammas, M. Theodoridou, A. Kramvis, P. Thiagarajan, Sharryn Gardner, G. Papaioannou, A. Melidou, M. Koutsaki, Georgia Kostagianni, Vassilis Achtsidis, C. Koutsaftiki, M. Calachanis, A. Zaravinos, A. Greenough, D. Spandidos   +14 more
semanticscholar   +1 more source

Comparison of the pathogenicity and neutrophil and monocyte response between SARS‐CoV‐2 prototype and Omicron BA.1 in a lethal mouse model

Animal Models and Experimental Medicine, EarlyView.
We conducted clinical characterization studies and performed virological evaluations on mice infected with SARS‐CoV‐2 prototype and Omicron BA.1 using RT‐PCR, HE and immunohistochemistry staining. We also studied the alterations of neutrophils and monocytes caused by the two strains using complete blood count, immunohistochemistry, and flow cytometry ...
Na Rong, Jing Wu, Binbin Zhao, Wanjun Peng, Hekai Yang, Gengxin Zhang, Dangting Ruan, Xiaohui Wei, Jiangning Liu   +8 more
wiley   +1 more source

Inference for stochastic kinetic models from multiple data sources for joint estimation of infection dynamics from aggregate reports and virological data [PDF]

arXiv, 2019
Influenza and respiratory syncytial virus (RSV) are the leading etiological agents of seasonal acute respiratory infections (ARI) around the world. Medical doctors typically base the diagnosis of ARI on patients' symptoms alone and do not always conduct virological tests necessary to identify individual viruses, which limits the ability to study the ...
arxiv