Results 131 to 140 of about 462,924 (342)

Functional CRISPR‐Cas9 knockout screening of the genetic determinants of human fibroblast migration propensity

open access: yesBiotechnology Progress, EarlyView.
Abstract Directional cell migration plays a central role in a wide range of physiological and pathological conditions, such as embryonic development or tumor metastasis. Steps involved in cell migration include cell polarization, formation of membrane protrusions at the cell front side and adhesion disassembly at the rear side, and a general ...
Antonio Mazzei   +5 more
wiley   +1 more source

Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic

open access: yesBiotechnology Progress, EarlyView.
Integrating non‐chromatographic operations, such as low pH viral inactivation and a depth filtration mimic, into an automated micro‐scale platform enhances productivity while reducing resource and time requirements. By facilitating rapid, data‐rich experimentation, the platform offers a powerful and efficient tool for assessing and optimizing ...
Paras Sharma   +4 more
wiley   +1 more source

Molecular studies on a complex of potyviruses infecting solanaceous crops, and some specific virus-host interactions [PDF]

open access: yes, 2003
This thesis constitutes a comprehensive analysis of the molecular and biological characteristics of three potyviruses (genus Potyvirus, family Potyviridae) naturally occurring in cultivated and wild species of family Solanaceae: Peru tomato virus (PTV ...
Spetz, Carl
core  

Enhanced deoxyviolacein bioproduction via enzyme condensation with a yeast glycolytic enzyme‐derived peptide tag

open access: yesBiotechnology Progress, EarlyView.
Fusion of a 27‐amino‐acid peptide tag derived from a glycolytic enzyme enabled artificial assembly formation of the two violacein biosynthetic enzymes, VioE and VioC. This assembly resulted in enhanced biosynthesis of deoxyviolacein. Abstract In microbial production, regulating endogenous and exogenous synthetic pathways is essential.
Rioko Ito   +4 more
wiley   +1 more source

Dynamic pH profiles drive higher cell‐specific and volumetric productivity

open access: yesBiotechnology Progress, EarlyView.
Implementation of dyamic pH profiles for increased CHO cell culture productivity. Abstract Mammalian cell cultures in bioreactors rely heavily on critical process parameter control to ensure optimal growth, productivity, and reproducibility to produce recombinant therapeutic proteins. Culture pH has been shown to be a critical parameter that influences
Stephanie R. Klaubert   +6 more
wiley   +1 more source

Virology, epidemiology, transmissions, diagnostic tests, prophylaxis and treatments of human Mpox: Saudi Arabia perspective

open access: yesFrontiers in Cellular and Infection Microbiology
Mpox (Monkeypox) is a highly contagious viral disease that can be transmitted from animal-to-human or human-to-human through intimate contact, Mpox is caused by the monkeypox virus (MPXV), which is an enveloped double-stranded DNA that belongs to the ...
Almonther Abdullah Hershan
doaj   +1 more source

Clinical and Virological Features of SARS-CoV-2 Variants of Concern: A Retrospective Cohort Study Comparing B.1.1.7 (Alpha), B.1.315 (Beta), and B.1.617.2 (Delta)

open access: green, 2021
Sean Wei Xiang Ong   +15 more
openalex   +1 more source

Image analysis method for measurement and prediction of intra‐matrix IgG diffusion

open access: yesBiotechnology Progress, EarlyView.
Experimental and image analysis workflow for b‐IgG diffusion in hyaluronic acid (HA) matrix. Abstract Measurement and imaging of intra‐matrix protein therapeutics diffusion is important due to the emergence of injectable biologics currently in various stages of research and clinical testing.
Riya Debbarma   +2 more
wiley   +1 more source

Optimizing sterile filtration of nanoemulsions through proper choice of prefilter properties

open access: yesBiotechnology Progress, EarlyView.
Abstract Nanoemulsions, with droplet sizes between 20 and 200 nm, have emerged as a promising vaccine adjuvant and drug delivery system, enhancing the solubility of hydrophobic drugs for diverse applications. Sterile filtration of nanoemulsions is particularly challenging due to the similar size between the nanodroplets and the 0.2 μm nominal pore size
Shreya Kapila   +4 more
wiley   +1 more source

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